BMRBj - BMREntryMaster

Found 1 Document (3.077 seconds)

BMRB: 11250

2RRE

Structure and function of the N-terminal nucleolin binding domain of nuclear valocine containing protein like 2 (NVL2) harboring a nucleolar localization signal.

Authors

Fujiwara, Y., Fujiwara, K., Goda, N., Iwaya, N., Tenno, T., Shirakawa, M., Hiroaki, H.

Assembly

nuclear valocine containing protein like 2 (NVL2)

Entity

1. nuclear valocine containing protein like 2 (NVL2) (polymer, Thiol state: all free), 74 monomers, 8694.021 Da Detail

MKPRPGVFVD RKLKQRVIQY LSSNRCGKYV DTGILASDLQ RLYSVDYGRR KRNAFRIQVE KVFSIISSEK ELKN

Formula weight

8694.021 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.2 %, Completeness (bb): 84.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.2 % (813 of 922)	92.9 % (456 of 491)	79.5 % (280 of 352)	97.5 % (77 of 79)
Backbone	84.1 % (370 of 440)	98.0 % (147 of 150)	70.2 % (153 of 218)	97.2 % (70 of 72)
Sidechain	92.8 % (512 of 552)	90.6 % (309 of 341)	96.1 % (196 of 204)	100.0 % (7 of 7)
Aromatic	93.5 % (58 of 62)	93.5 % (29 of 31)	93.5 % (29 of 31)
Methyl	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (39 of 39)

1. NVL2 N-terminal domain

MKPRPGVFVD RKLKQRVIQY LSSNRCGKYV DTGILASDLQ RLYSVDYGRR KRNAFRIQVE KVFSIISSEK ELKN

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	NVL2 N-terminal domain	[U-99% 15N]	protein	1.2 mM
2	sodium phosphate	natural abundance	buffer	25 mM
3	H2O	natural abundance	solvent	95 %
4	D2O	natural abundance	solvent	5 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
5	NVL2 N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.6 mM
6	sodium phosphate	natural abundance	buffer	25 mM
7	D2O	natural abundance	solvent	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	na	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	na	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	na	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RRE, Strand ID: A Detail

Release date

2011-06-23

Citation 1

Structure and function of the N-terminal nucleolin binding domain of nuclear valosin-containing protein-like 2 (NVL2) harboring a nucleolar localization signal
Fujiwara, Y., Fujiwara, K., Goda, N., Iwaya, N., Tenno, T., Shirakawa, M., Hiroaki, H.
J. Biol. Chem. (2011), 286, 21732-21741, PubMed 21474449 , DOI 10.1074/jbc.M110.174680 , Abstract

Show more 1 citaion

Citation 2

The PRESAT-vector: asymmetric T-vector for high-throughput screening of soluble protein domains for structural proteomics
Goda, N., Tenno, T., Takasu, H., Hiroaki, H., Shirakawa, M.
Protein Sci. (2004), 13, 652-658, PubMed 14978305 , DOI 10.1110/ps.03439004 , Abstract

Hide non-primary 1 citaion

Related entities 1. nuclear valocine containing protein like 2 (NVL2), : 1 : 2 : 7 entities Detail

Interaction partners 1. nuclear valocine containing protein like 2 (NVL2), : 6 interactors Detail

More details for 6 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	NVL2 N-terminal domain	[U-99% 15N]	protein	1.2 mM
2	sodium phosphate	natural abundance	buffer	25 mM
3	H2O	natural abundance	solvent	95 %
4	D2O	natural abundance	solvent	5 %

2 2D 1H-13C HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
5	NVL2 N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.6 mM
6	sodium phosphate	natural abundance	buffer	25 mM
7	D2O	natural abundance	solvent	100 %

3 3D HNCACB

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
5	NVL2 N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.6 mM
6	sodium phosphate	natural abundance	buffer	25 mM
7	D2O	natural abundance	solvent	100 %

4 3D H(CCO)NH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
5	NVL2 N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.6 mM
6	sodium phosphate	natural abundance	buffer	25 mM
7	D2O	natural abundance	solvent	100 %

5 3D 1H-15N NOESY

Instrument

Bruker DMX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	NVL2 N-terminal domain	[U-99% 15N]	protein	1.2 mM
2	sodium phosphate	natural abundance	buffer	25 mM
3	H2O	natural abundance	solvent	95 %
4	D2O	natural abundance	solvent	5 %

6 3D 1H-13C NOESY

Instrument

Bruker DMX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
5	NVL2 N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.6 mM
6	sodium phosphate	natural abundance	buffer	25 mM
7	D2O	natural abundance	solvent	100 %

7 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
5	NVL2 N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.6 mM
6	sodium phosphate	natural abundance	buffer	25 mM
7	D2O	natural abundance	solvent	100 %

nullKeywords AAA-ATPase, nucleolar localization signal, ribosomal biogenesis, alternative splicing, PROTEIN, RNA interaction

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (3.077 seconds)

BMRB: 11250

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. nuclear valocine containing protein like 2 (NVL2), : 1 : 2 : 7 entities Detail

Interaction partners 1. nuclear valocine containing protein like 2 (NVL2), : 6 interactors Detail

Experiments performed 7 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample