BMRB: 11251

The solution structure of the C-terminal region of Zinc finger FYVE domain-containing protein 21

Authors

Koshiba, S., Tomizawa, T., Hayashi, F., Tochio, N., Harada, T., Watanabe, S., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger FYVE domain-containing protein 21

Entity

1. Zinc finger FYVE domain-containing protein 21 (polymer, Thiol state: all free), 141 monomers, 15217.96 Da Detail

GSSGSSGAEF YDKQLKVLLS GATFLVTFGN SEKPETMTCR LSNNQRYLFL DGDSHYEIEI VHISTVQILT EGFPPGGGNA RATGMFLQYT VPGTEGVTQL KLTVVEDVTV GRRQAVAWLV AMHKAAKLLY ESRDQSGPSS G

Formula weight

15217.96 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 95.4 %, Completeness (bb): 93.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.4 % (1506 of 1579)	95.2 % (772 of 811)	96.0 % (595 of 620)	93.9 % (139 of 148)
Backbone	93.8 % (784 of 836)	92.5 % (271 of 293)	94.8 % (386 of 407)	93.4 % (127 of 136)
Sidechain	97.2 % (844 of 868)	96.7 % (501 of 518)	97.9 % (331 of 338)	100.0 % (12 of 12)
Aromatic	93.5 % (116 of 124)	93.5 % (58 of 62)	93.4 % (57 of 61)	100.0 % (1 of 1)
Methyl	100.0 % (162 of 162)	100.0 % (81 of 81)	100.0 % (81 of 81)

1. C-terminal region of ZFYVE21

GSSGSSGAEF YDKQLKVLLS GATFLVTFGN SEKPETMTCR LSNNQRYLFL DGDSHYEIEI VHISTVQILT EGFPPGGGNA RATGMFLQYT VPGTEGVTQL KLTVVEDVTV GRRQAVAWLV AMHKAAKLLY ESRDQSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	C-terminal region of ZFYVE21	[U-13C; U-15N]	protein	1.18 mM
2	TRIS	[U-2H]	buffer	20 mM
3	sodium chloride	natural abundance	salt	100 mM
4	DTT	[U-2H]	salt	1 mM
5	sodium azide	natural abundance	salt	0.02 %
6	D2O	[U-2H]	solvent	10 %
7	H2O	natural abundance	solvent	90 %

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RRF, Strand ID: A Detail

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Release date

2011-08-02

Citation

The solution structure of the C-terminal region of Zinc finger FYVE domain-containing protein 21
Koshiba, S., Tomizawa, T., Hayashi, F., Tochio, N., Harada, T., Watanabe, S., Kigawa, T., Yokoyama, S.

Related entities 1. Zinc finger FYVE domain-containing protein 21, : 1 : 2 : 6 entities Detail

Interaction partners 1. Zinc finger FYVE domain-containing protein 21, : 25 interactors Detail

Experiments performed 2 experiments Detail