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BMRB: 11252

2RRD

Resonance assignments of HRDC domain from Bloom syndrome protein

Authors

Sato, A., Mishima, M., Nagai, A., Kim, S., Ito, Y., Hakoshima, T., Jee, J., Kitano, K.

Assembly

HRDC domain from Bloom syndrome protein

Entity

1. HRDC domain from Bloom syndrome protein (polymer, Thiol state: all free), 101 monomers, 11267.81 Da Detail

GIPEFKQKAL VAKVSQREEM VKKCLGELTE VCKSLGKVFG VHYFNIFNTV TLKKLAESLS SDPEVLLQID GVTEDKLEKY GAEVISVLQK YSEWTSPAED S

Formula weight

11267.81 Da

Source organism

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.1 %, Completeness: 74.4 %, Completeness (bb): 76.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.4 % (890 of 1197)	78.8 % (490 of 622)	66.2 % (311 of 470)	84.8 % (89 of 105)
Backbone	76.7 % (460 of 600)	87.8 % (180 of 205)	64.3 % (191 of 297)	90.8 % (89 of 98)
Sidechain	74.6 % (516 of 692)	74.3 % (310 of 417)	76.9 % (206 of 268)	0.0 % (0 of 7)
Aromatic	30.0 % (24 of 80)	30.0 % (12 of 40)	30.8 % (12 of 39)	0.0 % (0 of 1)
Methyl	86.3 % (107 of 124)	85.5 % (53 of 62)	87.1 % (54 of 62)

1. BLM HRDC domain

GIPEFKQKAL VAKVSQREEM VKKCLGELTE VCKSLGKVFG VHYFNIFNTV TLKKLAESLS SDPEVLLQID GVTEDKLEKY GAEVISVLQK YSEWTSPAED S

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	BLM HRDC domain	[U-98% 13C; U-98% 15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	50 mM
3	sodium chloride	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	natural abundance	solvent	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
6	BLM HRDC domain	[U-100% 15N]	protein	1 mM
7	d11-TRIS	natural abundance	buffer	50 mM
8	sodium chloride	natural abundance	salt	100 mM
9	H2O	natural abundance	solvent	90 %
10	D2O	natural abundance	solvent	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
11	BLM HRDC domain	[U-98% 13C; U-98% 15N]	protein	1 mM
12	d11-TRIS	natural abundance	buffer	50 mM
13	sodium chloride	natural abundance	salt	100 mM
14	D2O	natural abundance	solvent	100 %

LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.12 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RRD, Strand ID: A Detail

Release date

2010-09-27

Citation

Resonance assignments of HRDC domain from Bloom syndrome protein
Sato, A., Mishima, M., Nagai, A., Kim, S., Ito, Y., Hakoshima, T., Jee, J., Kitano, K.
J. Biochem.

Related entities 1. HRDC domain from Bloom syndrome protein, : 1 : 6 : 12 entities Detail

Interaction partners 1. HRDC domain from Bloom syndrome protein, : 42 interactors Detail

More details for 42 interactors identified by 25 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	BLM HRDC domain	[U-98% 13C; U-98% 15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	50 mM
3	sodium chloride	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	natural abundance	solvent	10 %

2 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	BLM HRDC domain	[U-98% 13C; U-98% 15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	50 mM
3	sodium chloride	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	natural abundance	solvent	10 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	BLM HRDC domain	[U-98% 13C; U-98% 15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	50 mM
3	sodium chloride	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	natural abundance	solvent	10 %

4 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	BLM HRDC domain	[U-98% 13C; U-98% 15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	50 mM
3	sodium chloride	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	natural abundance	solvent	10 %

5 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	BLM HRDC domain	[U-98% 13C; U-98% 15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	50 mM
3	sodium chloride	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	natural abundance	solvent	10 %

6 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	BLM HRDC domain	[U-98% 13C; U-98% 15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	50 mM
3	sodium chloride	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	natural abundance	solvent	10 %

7 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	BLM HRDC domain	[U-98% 13C; U-98% 15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	50 mM
3	sodium chloride	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	natural abundance	solvent	10 %

8 4D H(CCO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	BLM HRDC domain	[U-98% 13C; U-98% 15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	50 mM
3	sodium chloride	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	natural abundance	solvent	10 %

9 3D HNHB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
6	BLM HRDC domain	[U-100% 15N]	protein	1 mM
7	d11-TRIS	natural abundance	buffer	50 mM
8	sodium chloride	natural abundance	salt	100 mM
9	H2O	natural abundance	solvent	90 %
10	D2O	natural abundance	solvent	10 %

10 3D HN(CO)HB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	BLM HRDC domain	[U-98% 13C; U-98% 15N]	protein	0.5 mM
2	TRIS	natural abundance	buffer	50 mM
3	sodium chloride	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	natural abundance	solvent	10 %

11 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
6	BLM HRDC domain	[U-100% 15N]	protein	1 mM
7	d11-TRIS	natural abundance	buffer	50 mM
8	sodium chloride	natural abundance	salt	100 mM
9	H2O	natural abundance	solvent	90 %
10	D2O	natural abundance	solvent	10 %

12 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
11	BLM HRDC domain	[U-98% 13C; U-98% 15N]	protein	1 mM
12	d11-TRIS	natural abundance	buffer	50 mM
13	sodium chloride	natural abundance	salt	100 mM
14	D2O	natural abundance	solvent	100 %

nullKeywords Bloom Syndrome Protein, DNA Helicase, HRDC Domain, RecQ Family