BMRB: 11253

Solution Structure of the Ubiquitin-like Domain from Mouse Hypothetical 1700011N24Rik Protein

Authors

Zhao, C., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

1700011N24Rik protein

Entity

1. 1700011N24Rik protein (polymer, Thiol state: all free), 102 monomers, 10986.24 Da Detail

GSSGSSGMLI TVYCVRRDLT EVTFSLQVNP DFELSNFRVL CELESGVPAE EAQIVYMEQL LTDDHCSLGS YGLKDGDMVV LLQKDNVGLR TPGRTPSGPS SG

Formula weight

10986.24 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.2 %, Completeness: 88.9 %, Completeness (bb): 86.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.9 % (994 of 1118)	88.9 % (512 of 576)	88.8 % (389 of 438)	89.4 % (93 of 104)
Backbone	86.5 % (521 of 602)	85.7 % (180 of 210)	86.4 % (255 of 295)	88.7 % (86 of 97)
Sidechain	91.8 % (557 of 607)	90.7 % (332 of 366)	93.2 % (218 of 234)	100.0 % (7 of 7)
Aromatic	93.1 % (54 of 58)	93.1 % (27 of 29)	93.1 % (27 of 29)
Methyl	97.4 % (113 of 116)	100.0 % (58 of 58)	94.8 % (55 of 58)

1. Ubiquitin-like Domain

GSSGSSGMLI TVYCVRRDLT EVTFSLQVNP DFELSNFRVL CELESGVPAE EAQIVYMEQL LTDDHCSLGS YGLKDGDMVV LLQKDNVGLR TPGRTPSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 6.0 (±0.05), Details 1.17mM Protein U-15N, {13C;} 20mM Phosphate Na {(pH6.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% {H2O;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin-like Domain	[U-13C; U-15N]	protein	1.17 mM
2	Phosphate Na	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.26 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V5O, Strand ID: A Detail

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Release date

2011-08-16

Citation

Solution Structure of the Ubiquitin-like Domain from Mouse Hypothetical 1700011N24Rik Protein
Zhao, C., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. 1700011N24Rik protein, : 1 : 188 entities Detail

Experiments performed 2 experiments Detail