BMRB: 11254

Solution Structure of a N-terminal Ubiquitin-like Domain in Mouse Tubulin-specific Chaperone B

Authors

Zhao, C., Kigawa, T., Saito, K., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

cytoskeleton-associated protein 1

Entity

1. cytoskeleton-associated protein 1 (polymer, Thiol state: all free), 95 monomers, 10161.22 Da Detail

GSSGSSGVMV FISSSLNSFR SEKRYSRSLT IAEFKCKLEL VVGSPASCME LELYGADDKF YSKLDQEDAL LGSYPVDDGC RIHVIDHSGS GPSSG

Formula weight

10161.22 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 97.3 %, Completeness (bb): 97.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.3 % (1007 of 1035)	96.1 % (513 of 534)	98.8 % (402 of 407)	97.9 % (92 of 94)
Backbone	97.9 % (552 of 564)	96.4 % (190 of 197)	98.9 % (272 of 275)	97.8 % (90 of 92)
Sidechain	97.1 % (540 of 556)	95.8 % (323 of 337)	99.1 % (215 of 217)	100.0 % (2 of 2)
Aromatic	95.0 % (76 of 80)	95.0 % (38 of 40)	95.0 % (38 of 40)
Methyl	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)

1. Ubiquitin-like domain

GSSGSSGVMV FISSSLNSFR SEKRYSRSLT IAEFKCKLEL VVGSPASCME LELYGADDKF YSKLDQEDAL LGSYPVDDGC RIHVIDHSGS GPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 6.0 (±0.05), Details 1.15mM Protein U-15N, {13C;} 20mM Phosphate Na {(pH6.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin-like domain	[U-13C; U-15N]	protein	1.15 mM
2	Phosphate Na	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.17 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.17 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V6E, Strand ID: A Detail

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Release date

2011-08-16

Citation

Solution Structure of a N-terminal Ubiquitin-like Domain in Mouse Tubulin-specific Chaperone B
Zhao, C., Kigawa, T., Saito, K., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. cytoskeleton-associated protein 1, : 1 : 2 : 26 entities Detail

Interaction partners 1. cytoskeleton-associated protein 1, : 4 interactors Detail

Experiments performed 2 experiments Detail