BMRB: 11255

Solution Structure of Anticodon Binding Domain from Nuclear Receptor Coactivator 5 (Human KIAA1637 Protein)

Authors

Zhao, C., Kigawa, T., Tochio, N., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Nuclear receptor coactivator 5

Entity

1. Nuclear receptor coactivator 5 (polymer, Thiol state: all free), 130 monomers, 14296.91 Da Detail

GSSGSSGPVD CSVIVVNKQT KDYAESVGRK VRDLGMVVDL IFLNTEVSLS QALEDVSRGG SPFAIVITQQ HQIHRSCTVN IMFGTPQEHR NMPQADAMVL VARNYERYKN ECREKEREEI ARQASGPSSG

Formula weight

14296.91 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.8 %, Completeness: 89.5 %, Completeness (bb): 87.9 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.5 % (1301 of 1454)	89.3 % (678 of 759)	90.5 % (503 of 556)	86.3 % (120 of 139)
Backbone	87.9 % (677 of 770)	87.9 % (233 of 265)	88.4 % (336 of 380)	86.4 % (108 of 125)
Sidechain	91.3 % (734 of 804)	90.1 % (445 of 494)	93.6 % (277 of 296)	85.7 % (12 of 14)
Aromatic	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)
Methyl	98.5 % (132 of 134)	98.5 % (66 of 67)	98.5 % (66 of 67)

1. Anticodon Binding Domain

GSSGSSGPVD CSVIVVNKQT KDYAESVGRK VRDLGMVVDL IFLNTEVSLS QALEDVSRGG SPFAIVITQQ HQIHRSCTVN IMFGTPQEHR NMPQADAMVL VARNYERYKN ECREKEREEI ARQASGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 7.5 (±0.05), Details 1.02mM Anticodon Binding Domain U-15N, {13C;} 20mM d-Tris-HCl {(pH7.5);} 200mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% {D2O;} 90% H2O

#	Name	Isotope labeling	Type	Concentration
1	Anticodon Binding Domain	[U-13C; U-15N]	protein	1.02 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	200 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.19 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.18 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1V95, Strand ID: A Detail

---

Release date

2011-08-16

Citation

Solution Structure of Anticodon Binding Domain from Nuclear Receptor Coactivator 5 (Human KIAA1637 Protein)
Zhao, C., Kigawa, T., Tochio, N., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Nuclear receptor coactivator 5, : 1 : 9 entities Detail

Interaction partners 1. Nuclear receptor coactivator 5, : 28 interactors Detail

Experiments performed 2 experiments Detail