BMRB: 11256

Solution Structure of the N-terminal Ubiquitin-like Domain of Mouse Ubiquitin Specific Protease 14 (USP14)

Authors

Zhao, C., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Ubiquitin carboxyl-terminal hydrolase 14

Entity

1. Ubiquitin carboxyl-terminal hydrolase 14 (polymer, Thiol state: not present), 96 monomers, 10110.40 Da Detail

GSSGSSGYSV TVKWGKEKFE GVELNTDEPP MVFKAQLFAL TGVQPARQKV MVKGGTLKDD DWGNIKMKNG MTVLMMGSAD ALPEEPSAKT SGPSSG

Formula weight

10110.4 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 98.2 %, Completeness (bb): 98.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.2 % (1047 of 1066)	97.7 % (543 of 556)	99.0 % (408 of 412)	98.0 % (96 of 98)
Backbone	98.4 % (555 of 564)	98.0 % (195 of 199)	98.9 % (272 of 275)	97.8 % (88 of 90)
Sidechain	98.3 % (575 of 585)	97.5 % (348 of 357)	99.5 % (219 of 220)	100.0 % (8 of 8)
Aromatic	96.8 % (60 of 62)	96.8 % (30 of 31)	96.6 % (28 of 29)	100.0 % (2 of 2)
Methyl	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (42 of 42)

1. ubiquitin-like domain

GSSGSSGYSV TVKWGKEKFE GVELNTDEPP MVFKAQLFAL TGVQPARQKV MVKGGTLKDD DWGNIKMKNG MTVLMMGSAD ALPEEPSAKT SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 6.0 (±0.05), Details 20mM PiNa {(pH6.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like domain		protein	0.0 ~ 0.0 mM
2	PiNa	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.18 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WGG, Strand ID: A Detail

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Release date

2011-08-16

Citation

Solution Structure of the N-terminal Ubiquitin-like Domain of Mouse Ubiquitin Specific Protease 14 (USP14)
Zhao, C., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Ubiquitin carboxyl-terminal hydrolase 14, : 1 : 4 : 59 entities Detail

Interaction partners 1. Ubiquitin carboxyl-terminal hydrolase 14, : 2 interactors Detail

Experiments performed 2 experiments Detail