BMRB: 11257

Solution Structure of Mouse Ubiquitin-like 3 Protein

Authors

Zhao, C., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

ubiquitin-like 3

Entity

1. ubiquitin-like 3 (polymer, Thiol state: not present), 116 monomers, 12624.09 Da Detail

GSSGSSGMSS HVPADMINLR LILVSGKTKE FLFSPNDSAS DIAKHVYDNW PMDWEEEQVS SPNILRLIYQ GRFLHGNVTL GALKLPFGKT TVMHLVARET LPEPNSQGQR SGPSSG

Formula weight

12624.09 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.0 %, Completeness: 87.5 %, Completeness (bb): 88.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.5 % (1152 of 1317)	87.3 % (598 of 685)	88.3 % (452 of 512)	85.0 % (102 of 120)
Backbone	88.5 % (602 of 680)	90.2 % (212 of 235)	88.1 % (297 of 337)	86.1 % (93 of 108)
Sidechain	86.8 % (644 of 742)	85.8 % (386 of 450)	88.9 % (249 of 280)	75.0 % (9 of 12)
Aromatic	95.8 % (92 of 96)	97.9 % (47 of 48)	95.7 % (44 of 46)	50.0 % (1 of 2)
Methyl	96.6 % (112 of 116)	98.3 % (57 of 58)	94.8 % (55 of 58)

1. Ubiquitin-like Fold

GSSGSSGMSS HVPADMINLR LILVSGKTKE FLFSPNDSAS DIAKHVYDNW PMDWEEEQVS SPNILRLIYQ GRFLHGNVTL GALKLPFGKT TVMHLVARET LPEPNSQGQR SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 6.0 (±0.05), Details 20mM PiNa {(pH6.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% {H2O;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin-like Fold		protein	0.0 ~ 0.0 mM
2	PiNa	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.23 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WGH, Strand ID: A Detail

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Release date

2011-08-16

Citation

Solution Structure of Mouse Ubiquitin-like 3 Protein
Zhao, C., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. ubiquitin-like 3, : 1 : 14 entities Detail

Experiments performed 2 experiments Detail