BMRB: 11258

Solution Structure of Mouse Hypothetical Protein 2900073H19RIK

Authors

Zhao, C., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

RIKEN cDNA 2900073H19 protein

Entity

1. RIKEN cDNA 2900073H19 protein (polymer, Thiol state: all free), 114 monomers, 12314.84 Da Detail

GSSGSSGMAA PLCVKVEFGG GAELLFDGVK KHQVALPGQE EPWDIRNLLV WIKKNLLKER PELFIQGDSV RPGILVLIND ADWELLGELD YQLQDQDSIL FISTLHGGSG PSSG

Formula weight

12314.84 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.2 %, Completeness: 93.2 %, Completeness (bb): 89.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.2 % (1219 of 1308)	92.5 % (628 of 679)	94.7 % (482 of 509)	90.8 % (109 of 120)
Backbone	89.7 % (603 of 672)	86.5 % (205 of 237)	92.0 % (301 of 327)	89.8 % (97 of 108)
Sidechain	96.6 % (710 of 735)	95.7 % (423 of 442)	97.9 % (275 of 281)	100.0 % (12 of 12)
Aromatic	97.8 % (90 of 92)	97.8 % (45 of 46)	97.7 % (42 of 43)	100.0 % (3 of 3)
Methyl	100.0 % (136 of 136)	100.0 % (68 of 68)	100.0 % (68 of 68)

1. ThiS domain

GSSGSSGMAA PLCVKVEFGG GAELLFDGVK KHQVALPGQE EPWDIRNLLV WIKKNLLKER PELFIQGDSV RPGILVLIND ADWELLGELD YQLQDQDSIL FISTLHGGSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 6.0 (±0.05), Details 20mM PiNa {(pH6.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ThiS domain		protein	0.0 ~ 0.0 mM
2	PiNa	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.51 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WGK, Strand ID: A Detail

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Release date

2011-08-16

Citation

Solution Structure of Mouse Hypothetical Protein 2900073H19RIK
Zhao, C., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. RIKEN cDNA 2900073H19 protein, : 1 : 3 : 74 entities Detail

Interaction partners 1. RIKEN cDNA 2900073H19 protein, : 12 interactors Detail

Experiments performed 2 experiments Detail