BMRB: 11259

Solution Structure of CUE domain in the C-terminal of Human Toll-interacting Protein (Tollip)

Authors

Zhao, C., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Toll-interacting protein

Entity

1. Toll-interacting protein (polymer, Thiol state: all free), 59 monomers, 6156.650 Da Detail

GSSGSSGCSE EDLKAIQDMF PNMDQEVIRS VLEAQRGNKD AAINSLLQMG EEPSGPSSG

Formula weight

6156.65 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.8 %, Completeness: 90.0 %, Completeness (bb): 87.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.0 % (560 of 622)	88.7 % (290 of 327)	91.8 % (213 of 232)	90.5 % (57 of 63)
Backbone	87.1 % (303 of 348)	82.8 % (101 of 122)	89.4 % (152 of 170)	89.3 % (50 of 56)
Sidechain	93.3 % (304 of 326)	92.2 % (189 of 205)	94.7 % (108 of 114)	100.0 % (7 of 7)
Aromatic	100.0 % (10 of 10)	100.0 % (5 of 5)	100.0 % (5 of 5)
Methyl	97.7 % (43 of 44)	95.5 % (21 of 22)	100.0 % (22 of 22)

1. CUE domain

GSSGSSGCSE EDLKAIQDMF PNMDQEVIRS VLEAQRGNKD AAINSLLQMG EEPSGPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 7.0 (±0.05), Details 20mM d-Tris-HCl {(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CUE domain		protein	0.0 ~ 0.0 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WGL, Strand ID: A Detail

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Release date

2011-08-16

Citation

Solution Structure of CUE domain in the C-terminal of Human Toll-interacting Protein (Tollip)
Zhao, C., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Toll-interacting protein, : 1 : 16 entities Detail

Experiments performed 2 experiments Detail