BMRB: 11260

Solution Structure of UBA domain of Human Ubiquitin Associated Protein 1 (UBAP1)

Authors

Zhao, C., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

ubiquitin associated protein

Entity

1. ubiquitin associated protein (polymer, Thiol state: all free), 63 monomers, 6808.554 Da Detail

GSSGSSGAYS ELQMLSPSER QCVETVVNMG YSYECVLRAM KKKGENIEQI LDYLFAHSGP SSG

Formula weight

6808.554 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.9 %, Completeness: 91.8 %, Completeness (bb): 88.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.8 % (637 of 694)	92.0 % (332 of 361)	92.5 % (247 of 267)	87.9 % (58 of 66)
Backbone	88.0 % (329 of 374)	87.0 % (114 of 131)	89.0 % (162 of 182)	86.9 % (53 of 61)
Sidechain	95.5 % (359 of 376)	94.8 % (218 of 230)	96.5 % (136 of 141)	100.0 % (5 of 5)
Aromatic	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (23 of 23)
Methyl	100.0 % (52 of 52)	100.0 % (26 of 26)	100.0 % (26 of 26)

1. UBA domain

GSSGSSGAYS ELQMLSPSER QCVETVVNMG YSYECVLRAM KKKGENIEQI LDYLFAHSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 7.0 (±0.05), Details 20mM d-Tris-HCl {(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	UBA domain		protein	0.0 ~ 0.0 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.45 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WGN, Strand ID: A Detail

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Release date

2011-08-16

Citation

Solution Structure of UBA domain of Human Ubiquitin Associated Protein 1 (UBAP1)
Zhao, C., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. ubiquitin associated protein, : 1 : 26 entities Detail

Experiments performed 2 experiments Detail