BMRB: 11261

Solution Structure of the N-terminal Ubiquitin-like Domain in the Human Ubiquilin 3 (UBQLN3)

Authors

Zhao, C., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Ubiquilin 3

Entity

1. Ubiquilin 3 (polymer, Thiol state: all free), 106 monomers, 11333.75 Da Detail

GSSGSSGSPA PVQDPHLIKV TVKTPKDKED FSVTDTCTIQ QLKEEISQRF KAHPDQLVLI FAGKILKDPD SLAQCGVRDG LTVHLVIKRQ HRAMGNECPA SGPSSG

Formula weight

11333.75 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.3 %, Completeness: 93.6 %, Completeness (bb): 92.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.6 % (1111 of 1187)	92.6 % (575 of 621)	95.0 % (437 of 460)	93.4 % (99 of 106)
Backbone	92.4 % (573 of 620)	90.1 % (192 of 213)	93.9 % (290 of 309)	92.9 % (91 of 98)
Sidechain	95.0 % (631 of 664)	93.9 % (383 of 408)	96.8 % (240 of 248)	100.0 % (8 of 8)
Aromatic	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (23 of 23)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. ubiquitin-like fold

GSSGSSGSPA PVQDPHLIKV TVKTPKDKED FSVTDTCTIQ QLKEEISQRF KAHPDQLVLI FAGKILKDPD SLAQCGVRDG LTVHLVIKRQ HRAMGNECPA SGPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 7.0 (±0.05), Details 2.0mM protein U-15N, 13C/20mM d-Tris-HCl (pH7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like fold	[U-13C; U-15N]	protein	2.0 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.23 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WX7, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of the N-terminal Ubiquitin-like Domain in the Human Ubiquilin 3 (UBQLN3)
Zhao, C., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Ubiquilin 3, : 1 : 29 entities Detail

Experiments performed 2 experiments Detail