BMRB: 11262

Solution Structure of the N-terminal Ubiquitin-like Domain in the 4931431F19Rik Protein

Authors

Zhao, C., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

RIKEN cDNA 4931431F19

Entity

1. RIKEN cDNA 4931431F19 (polymer, Thiol state: all free), 96 monomers, 10508.76 Da Detail

GSSGSSGVSG REPSSRIIRV SVKTPQDCHE FFLAENSNVR RFKKQISKYL HCNADRLVLI FTGKILRDQD ILSQRGILDG STVHVVVRSH SGPSSG

Formula weight

10508.76 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.5 %, Completeness: 87.8 %, Completeness (bb): 84.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.8 % (955 of 1088)	87.3 % (496 of 568)	89.0 % (374 of 420)	85.0 % (85 of 100)
Backbone	84.4 % (481 of 570)	82.8 % (164 of 198)	86.0 % (240 of 279)	82.8 % (77 of 93)
Sidechain	89.4 % (541 of 605)	88.6 % (328 of 370)	90.4 % (206 of 228)	100.0 % (7 of 7)
Aromatic	78.1 % (50 of 64)	78.1 % (25 of 32)	78.1 % (25 of 32)
Methyl	96.1 % (98 of 102)	96.1 % (49 of 51)	96.1 % (49 of 51)

1. ubiquitin-like domain

GSSGSSGVSG REPSSRIIRV SVKTPQDCHE FFLAENSNVR RFKKQISKYL HCNADRLVLI FTGKILRDQD ILSQRGILDG STVHVVVRSH SGPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 7.0 (±0.05), Details 1.51mM protein U-15N, 13C, 20mM d-Tris-HCl (pH7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like domain	[U-13C; U-15N]	protein	1.51 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.09 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.09 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.13 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WX8, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of the N-terminal Ubiquitin-like Domain in the 4931431F19Rik Protein
Zhao, C., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. RIKEN cDNA 4931431F19, : 1 : 1 : 78 entities Detail

Experiments performed 2 experiments Detail