BMRB: 11265

Solution Structure of the N-terminal Ras-binding Domain (RBD) in Human a-Raf Kinase

Authors

Zhao, C., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

A-Raf proto-oncogene serine/threonine-protein kinase

Entity

1. A-Raf proto-oncogene serine/threonine-protein kinase (polymer, Thiol state: all free), 86 monomers, 9114.315 Da Detail

GSSGSSGGTV KVYLPNKQRT VVTVRDGMSV YDSLDKALKV RGLNQDCCVV YRLIKGRKTV TAWDTAIAPL DGEELIVEVL SGPSSG

Formula weight

9114.315 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.9 %, Completeness: 91.8 %, Completeness (bb): 88.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.8 % (864 of 941)	91.1 % (441 of 484)	93.5 % (345 of 369)	88.6 % (78 of 88)
Backbone	88.8 % (453 of 510)	86.6 % (155 of 179)	90.7 % (225 of 248)	88.0 % (73 of 83)
Sidechain	95.3 % (483 of 507)	93.8 % (286 of 305)	97.5 % (192 of 197)	100.0 % (5 of 5)
Aromatic	100.0 % (36 of 36)	100.0 % (18 of 18)	100.0 % (17 of 17)	100.0 % (1 of 1)
Methyl	99.1 % (111 of 112)	100.0 % (56 of 56)	98.2 % (55 of 56)

1. Ras-binding domain

GSSGSSGGTV KVYLPNKQRT VVTVRDGMSV YDSLDKALKV RGLNQDCCVV YRLIKGRKTV TAWDTAIAPL DGEELIVEVL SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296.0 (±0.1) K, pH 7.0 (±0.05), Details 0.86mM protein U-15N, {13C;} 20mM d-Tris-HCl {(pH7.0);} 200mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Ras-binding domain	[U-13C; U-15N]	protein	0.86 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	200 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.49 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WXM, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of the N-terminal Ras-binding Domain (RBD) in Human a-Raf Kinase
Zhao, C., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. A-Raf proto-oncogene serine/threonine-protein kinase, : 1 : 6 : 73 entities Detail

Interaction partners 1. A-Raf proto-oncogene serine/threonine-protein kinase, : 59 interactors Detail

Experiments performed 2 experiments Detail