BMRB: 11267

Solution Structure of Human SUMO-2 (SMT3B), a Ubiquitin-like Protein

Authors

Zhao, C., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Ubiquitin-like protein SMT3B

Entity

1. Ubiquitin-like protein SMT3B (polymer, Thiol state: all free), 104 monomers, 11486.60 Da Detail

GSSGSSGMAD EKPKEGVKTE NNDHINLKVA GQDGSVVQFK IKRHTPLSKL MKAYCERQGL SMRQIRFRFD GQPINETDTP AQLEMEDEDT IDVFQQQTSG PSSG

Formula weight

11486.6 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.5 %, Completeness: 88.3 %, Completeness (bb): 86.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.3 % (1044 of 1182)	88.0 % (550 of 625)	89.7 % (399 of 445)	84.8 % (95 of 112)
Backbone	86.0 % (528 of 614)	84.5 % (180 of 213)	87.4 % (264 of 302)	84.8 % (84 of 99)
Sidechain	90.6 % (600 of 662)	89.8 % (370 of 412)	92.4 % (219 of 237)	84.6 % (11 of 13)
Aromatic	100.0 % (56 of 56)	100.0 % (28 of 28)	100.0 % (28 of 28)
Methyl	100.0 % (80 of 80)	100.0 % (40 of 40)	100.0 % (40 of 40)

1. ubiquitin-like molecule

GSSGSSGMAD EKPKEGVKTE NNDHINLKVA GQDGSVVQFK IKRHTPLSKL MKAYCERQGL SMRQIRFRFD GQPINETDTP AQLEMEDEDT IDVFQQQTSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 7.0 (±0.05), Details 1.21mM protein U-15N, {13C;} 20mM d-Tris-HCl {(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like molecule	[U-13C; U-15N]	protein	1.21 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.11 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WZ0, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of Human SUMO-2 (SMT3B), a Ubiquitin-like Protein
Zhao, C., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Ubiquitin-like protein SMT3B, : 1 : 18 : 71 entities Detail

Interaction partners 1. Ubiquitin-like protein SMT3B, : 2 interactors Detail

Experiments performed 2 experiments Detail