BMRB: 11268

Solution Structure of the N-terminal Ubiquitin-like Domain in Mouse Ubiquitin-like Protein SB132

Authors

Zhao, C., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Ubiquitin-like protein SB132

Entity

1. Ubiquitin-like protein SB132 (polymer, Thiol state: all free), 107 monomers, 11506.90 Da Detail

GSSGSSGMSL SDWHLAVKLA DQPLAPKSIL QLPETELGEY SLGGYSISFL KQLIAGKLQE SVPDPELIDL IYCGRKLKDD QTLDFYGIQP GSTVHVLRKS WSGPSSG

Formula weight

11506.9 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.5 %, Completeness: 94.3 %, Completeness (bb): 92.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.3 % (1150 of 1220)	93.8 % (595 of 634)	95.2 % (455 of 478)	92.6 % (100 of 108)
Backbone	92.5 % (581 of 628)	90.4 % (198 of 219)	94.2 % (291 of 309)	92.0 % (92 of 100)
Sidechain	96.1 % (660 of 687)	95.7 % (397 of 415)	96.6 % (255 of 264)	100.0 % (8 of 8)
Aromatic	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (40 of 40)	100.0 % (2 of 2)
Methyl	98.3 % (116 of 118)	100.0 % (59 of 59)	96.6 % (57 of 59)

1. ubiquitin-like domain

GSSGSSGMSL SDWHLAVKLA DQPLAPKSIL QLPETELGEY SLGGYSISFL KQLIAGKLQE SVPDPELIDL IYCGRKLKDD QTLDFYGIQP GSTVHVLRKS WSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 7.0 (±0.05), Details 1.35mM protein U-15N, {13C;} 20mM d-Tris-HCl {(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like domain	[U-13C; U-15N]	protein	1.35 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.24 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X1M, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of the N-terminal Ubiquitin-like Domain in Mouse Ubiquitin-like Protein SB132
Zhao, C., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Ubiquitin-like protein SB132, : 1 : 3 : 11 entities Detail

Interaction partners 1. Ubiquitin-like protein SB132, : 1 interactors Detail

Experiments performed 2 experiments Detail