BMRB: 11269

Solution Structure of the N-terminal CUE Domain in the Human Mitogen-activated Protein Kinase Kinase Kinase 7 Interacting Protein 2 (MAP3K7IP2)

Authors

Zhao, C., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

KIAA0733 protein

Entity

1. KIAA0733 protein (polymer, Thiol state: all free), 75 monomers, 8027.668 Da Detail

GSSGSSGQID FQVLHDLRQK FPEVPEVVVS RCMLQNNNNL DACCAVLSQE STRYLYGEGD LNFSDDSGIS GPSSG

Formula weight

8027.668 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 90.5 %, Completeness (bb): 90.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.5 % (732 of 809)	88.5 % (371 of 419)	92.2 % (284 of 308)	93.9 % (77 of 82)
Backbone	90.3 % (401 of 444)	87.1 % (135 of 155)	91.7 % (199 of 217)	93.1 % (67 of 72)
Sidechain	91.2 % (394 of 432)	89.4 % (236 of 264)	93.7 % (148 of 158)	100.0 % (10 of 10)
Aromatic	84.0 % (42 of 50)	84.0 % (21 of 25)	84.0 % (21 of 25)
Methyl	97.0 % (64 of 66)	93.9 % (31 of 33)	100.0 % (33 of 33)

1. CUE domain

GSSGSSGQID FQVLHDLRQK FPEVPEVVVS RCMLQNNNNL DACCAVLSQE STRYLYGEGD LNFSDDSGIS GPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296.0 (±0.1) K, pH 7.0 (±0.05), Details 1.30mM CUE domain U-15N, {13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10%D2O

#	Name	Isotope labeling	Type	Concentration
1	CUE domain	[U-13C; U-15N]	protein	1.30 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.02 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DAE, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of the N-terminal CUE Domain in the Human Mitogen-activated Protein Kinase Kinase Kinase 7 Interacting Protein 2 (MAP3K7IP2)
Zhao, C., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. KIAA0733 protein, : 1 : 2 : 6 entities Detail

Interaction partners 1. KIAA0733 protein, : 63 interactors Detail

Experiments performed 2 experiments Detail