BMRB: 11270

Solution Structure of the Novel Identified Ubiquitin-like Domain in the Human Hypothetical Protein FLJ35834

Authors

Zhao, C., Kigawa, T., Saito, K., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

FLJ35834 protein

Entity

1. FLJ35834 protein (polymer, Thiol state: not present), 118 monomers, 12804.49 Da Detail

GSSGSSGQES VEDSLATVKV VLIPVGQEIV IPFKVDTILK YLKDHFSHLL GIPHSVLQIR YSGKILKNNE TLVQHGVKPQ EIVQVEIFST NPDLYPVRRI DGLTDVSQII TVSGPSSG

Formula weight

12804.49 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 91.2 %, Completeness (bb): 91.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.2 % (1243 of 1363)	89.5 % (630 of 704)	93.3 % (502 of 538)	91.7 % (111 of 121)
Backbone	91.9 % (638 of 694)	90.0 % (215 of 239)	92.7 % (319 of 344)	93.7 % (104 of 111)
Sidechain	91.0 % (707 of 777)	89.2 % (415 of 465)	94.4 % (285 of 302)	70.0 % (7 of 10)
Aromatic	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)
Methyl	96.4 % (160 of 166)	96.4 % (80 of 83)	96.4 % (80 of 83)

1. ubiquitin-like domain

GSSGSSGQES VEDSLATVKV VLIPVGQEIV IPFKVDTILK YLKDHFSHLL GIPHSVLQIR YSGKILKNNE TLVQHGVKPQ EIVQVEIFST NPDLYPVRRI DGLTDVSQII TVSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 7.0 (±0.05), Details 1.29mM ubiquitin-like domain U-15N, {13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like domain	[U-13C; U-15N]	protein	1.29 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.26 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DAF, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of the Novel Identified Ubiquitin-like Domain in the Human Hypothetical Protein FLJ35834
Zhao, C., Kigawa, T., Saito, K., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. FLJ35834 protein, : 1 : 13 entities Detail

Interaction partners 1. FLJ35834 protein, : 16 interactors Detail

Experiments performed 2 experiments Detail