BMRB: 11271

Solution Structure of the C-terminal UBA Domain in the Human Ubiquilin 3

Authors

Zhao, C., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Ubiquilin-3

Entity

1. Ubiquilin-3 (polymer, Thiol state: not present), 54 monomers, 5547.008 Da Detail

GSSGSSGHFQ VQLEQLRSMG FLNREANLQA LIATGGDVDA AVEKLRQSSG PSSG

Formula weight

5547.008 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.9 %, Completeness: 90.4 %, Completeness (bb): 87.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.4 % (516 of 571)	89.5 % (265 of 296)	92.1 % (198 of 215)	88.3 % (53 of 60)
Backbone	87.0 % (280 of 322)	82.6 % (95 of 115)	90.3 % (139 of 154)	86.8 % (46 of 53)
Sidechain	94.6 % (279 of 295)	93.9 % (170 of 181)	95.3 % (102 of 107)	100.0 % (7 of 7)
Aromatic	83.3 % (20 of 24)	83.3 % (10 of 12)	83.3 % (10 of 12)
Methyl	100.0 % (52 of 52)	100.0 % (26 of 26)	100.0 % (26 of 26)

1. UBA domain

GSSGSSGHFQ VQLEQLRSMG FLNREANLQA LIATGGDVDA AVEKLRQSSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296.0 (±0.1) K, pH 7.0 (±0.05), Details 0.80mM UBA domain U-15N, {13C;} 20mM d-Tris-HCl (pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	UBA domain	[U-13C; U-15N]	protein	0.80 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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Calculated from 20 models in PDB: 2DAH, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of the C-terminal UBA Domain in the Human Ubiquilin 3
Zhao, C., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Ubiquilin-3, : 1 : 24 entities Detail

Experiments performed 2 experiments Detail