BMRB: 11272

Solution Structure of the Novel Identified Ubiquitin-like Domain in the Human COBL-like 1 Protein

Authors

Zhao, C., Kigawa, T., Saito, K., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

KIAA0977 protein

Entity

1. KIAA0977 protein (polymer, Thiol state: all free), 91 monomers, 9974.235 Da Detail

GSSGSSGEKT VRVVINFKKT QKTIVRVSPH ASLQELAPII CSKCEFDPLH TLLLKDYQSQ EPLDLTKSLN DLGLRELYAM DVNRESGPSS G

Formula weight

9974.235 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.2 %, Completeness: 93.4 %, Completeness (bb): 90.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.4 % (974 of 1043)	93.0 % (507 of 545)	94.8 % (384 of 405)	89.2 % (83 of 93)
Backbone	90.1 % (483 of 536)	88.0 % (161 of 183)	92.1 % (246 of 267)	88.4 % (76 of 86)
Sidechain	96.5 % (571 of 592)	95.6 % (346 of 362)	97.8 % (218 of 223)	100.0 % (7 of 7)
Aromatic	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (22 of 22)
Methyl	100.0 % (104 of 104)	100.0 % (52 of 52)	100.0 % (52 of 52)

1. ubiquitin-like domain

GSSGSSGEKT VRVVINFKKT QKTIVRVSPH ASLQELAPII CSKCEFDPLH TLLLKDYQSQ EPLDLTKSLN DLGLRELYAM DVNRESGPSS G

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Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 7.0 (±0.05), Details 1.14mM ubiquitin-like domain U-15N, {13C;} 20mM d-Tris-HCl (pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like domain	[U-13C; U-15N]	protein	1.14 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DAJ, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of the Novel Identified Ubiquitin-like Domain in the Human COBL-like 1 Protein
Zhao, C., Kigawa, T., Saito, K., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. KIAA0977 protein, : 1 : 2 : 18 entities Detail

Interaction partners 1. KIAA0977 protein, : 22 interactors Detail

Experiments performed 2 experiments Detail