BMRB: 11273

Solution Structure of the Novel Identified UBA-like Domain in the N-terminal of Human Fas Associated Factor 1 Protein

Authors

Zhao, C., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Protein KIAA0794

Entity

1. Protein KIAA0794 (polymer, Thiol state: all free), 62 monomers, 5951.484 Da Detail

GSSGSSGGSA ASSALKGLIQ QFTTITGASE SVGKHMLEAC NNNLEMAVTM FLDGGGSGPS SG

Formula weight

5951.484 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.3 %, Completeness: 89.5 %, Completeness (bb): 86.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.5 % (545 of 609)	89.0 % (275 of 309)	91.5 % (214 of 234)	84.8 % (56 of 66)
Backbone	86.5 % (320 of 370)	84.4 % (114 of 135)	89.1 % (155 of 174)	83.6 % (51 of 61)
Sidechain	94.1 % (272 of 289)	92.5 % (161 of 174)	96.4 % (106 of 110)	100.0 % (5 of 5)
Aromatic	100.0 % (24 of 24)	100.0 % (12 of 12)	100.0 % (12 of 12)
Methyl	100.0 % (56 of 56)	100.0 % (28 of 28)	100.0 % (28 of 28)

1. UBA-like domain

GSSGSSGGSA ASSALKGLIQ QFTTITGASE SVGKHMLEAC NNNLEMAVTM FLDGGGSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296.0 (±0.1) K, pH 7.0 (±0.05), Details 0.26mM UBA-like domain U-15N, {13C;} 20mM d-Tris-HCl (pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;90%} H2O, 10%D2O

#	Name	Isotope labeling	Type	Concentration
1	UBA-like domain	[U-13C; U-15N]	protein	0.26 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DAL, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of the Novel Identified UBA-like Domain in the N-terminal of Human Fas Associated Factor 1 Protein
Zhao, C., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Protein KIAA0794, : 1 : 8 entities Detail

Interaction partners 1. Protein KIAA0794, : 15 interactors Detail

Experiments performed 2 experiments Detail