BMRB: 11274

Solution Structure of the Novel Identified UBA-like Domain in the N-terminal of Human ETEA Protein

Authors

Zhao, C., Kigawa, T., Yoneyama, M., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

ETEA protein

Entity

1. ETEA protein (polymer, Thiol state: all free), 67 monomers, 7358.710 Da Detail

GSSGSSGAPE ERDLTQEQTE KLLQFQDLTG IESMDQCRHT LEQHNWNIEA AVQDRLNEQE GSGPSSG

Formula weight

7358.71 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.0 %, Completeness: 90.6 %, Completeness (bb): 88.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.6 % (662 of 731)	88.8 % (341 of 384)	93.7 % (253 of 270)	88.3 % (68 of 77)
Backbone	88.4 % (352 of 398)	84.2 % (117 of 139)	92.3 % (179 of 194)	86.2 % (56 of 65)
Sidechain	93.4 % (367 of 393)	91.4 % (224 of 245)	96.3 % (131 of 136)	100.0 % (12 of 12)
Aromatic	86.7 % (26 of 30)	86.7 % (13 of 15)	85.7 % (12 of 14)	100.0 % (1 of 1)
Methyl	100.0 % (50 of 50)	100.0 % (25 of 25)	100.0 % (25 of 25)

1. UBA-like domain

GSSGSSGAPE ERDLTQEQTE KLLQFQDLTG IESMDQCRHT LEQHNWNIEA AVQDRLNEQE GSGPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298.0 (±0.1) K, pH 7.0 (±0.05), Details 1.02mM UBA-like domain U-15N, {13C;} 20mM d-Tris-HCl (pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	UBA-like domain	[U-13C; U-15N]	protein	1.02 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.4 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.4 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.74 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DAM, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of the Novel Identified UBA-like Domain in the N-terminal of Human ETEA Protein
Zhao, C., Kigawa, T., Yoneyama, M., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. ETEA protein, : 1 : 3 : 18 entities Detail

Interaction partners 1. ETEA protein, : 42 interactors Detail

Experiments performed 2 experiments Detail