BMRB: 11275

Solution Structure of the RRM Domain in the Human Poly (ADP-ribose) Polymerase Family, Member 10 Variant

Authors

Zhao, C., Kigawa, T., Tochio, N., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

poly (ADP-ribose) polymerase family, member 10 variant

Entity

1. poly (ADP-ribose) polymerase family, member 10 variant (polymer, Thiol state: all free), 104 monomers, 10701.94 Da Detail

GSSGSSGGVA VEVRGLPPAV PDELLTLYFE NRRRSGGGPV LSWQRLGCGG VLTFREPADA ERVLAQADHE LHGAQLSLRP APPRAPARLL LQGLPPGTSG PSSG

Formula weight

10701.94 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.2 %, Completeness: 92.0 %, Completeness (bb): 91.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.0 % (1026 of 1115)	90.0 % (524 of 582)	94.5 % (411 of 435)	92.9 % (91 of 98)
Backbone	91.7 % (550 of 600)	89.6 % (190 of 212)	92.9 % (275 of 296)	92.4 % (85 of 92)
Sidechain	92.7 % (559 of 603)	90.3 % (334 of 370)	96.5 % (219 of 227)	100.0 % (6 of 6)
Aromatic	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (23 of 23)	100.0 % (1 of 1)
Methyl	93.9 % (107 of 114)	91.2 % (52 of 57)	96.5 % (55 of 57)

1. RRM domain

GSSGSSGGVA VEVRGLPPAV PDELLTLYFE NRRRSGGGPV LSWQRLGCGG VLTFREPADA ERVLAQADHE LHGAQLSLRP APPRAPARLL LQGLPPGTSG PSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296.0 (±0.1) K, pH 7.0 (±0.05), Details 1.22mM RRM domain U-15N, {13C;} 20mM d-Tris-HCl {(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10%D2O

#	Name	Isotope labeling	Type	Concentration
1	RRM domain	[U-13C; U-15N]	protein	1.22 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.26 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DHX, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of the RRM Domain in the Human Poly (ADP-ribose) Polymerase Family, Member 10 Variant
Zhao, C., Kigawa, T., Tochio, N., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. poly (ADP-ribose) polymerase family, member 10 variant, : 1 : 13 entities Detail

Interaction partners 1. poly (ADP-ribose) polymerase family, member 10 variant, : 2 interactors Detail

Experiments performed 2 experiments Detail