BMRB: 11276

Solution Structure of the CUE Domain in the Human CUE Domain Containing Protein 1 (CUEDC1)

Authors

Zhao, C., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

CUE domain-containing protein 1

Entity

1. CUE domain-containing protein 1 (polymer, Thiol state: all free), 67 monomers, 7297.056 Da Detail

GSSGSSGRPA RQVRRLEFNQ AMDDFKTMFP NMDYDIIECV LRANSGAVDA TIDQLLQMNL ESGPSSG

Formula weight

7297.056 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.0 %, Completeness: 92.1 %, Completeness (bb): 91.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.1 % (679 of 737)	90.1 % (347 of 385)	94.6 % (265 of 280)	93.1 % (67 of 72)
Backbone	91.2 % (361 of 396)	88.3 % (121 of 137)	92.8 % (181 of 195)	92.2 % (59 of 64)
Sidechain	93.5 % (376 of 402)	91.1 % (226 of 248)	97.3 % (142 of 146)	100.0 % (8 of 8)
Aromatic	100.0 % (38 of 38)	100.0 % (19 of 19)	100.0 % (19 of 19)
Methyl	96.6 % (56 of 58)	96.6 % (28 of 29)	96.6 % (28 of 29)

1. CUE domain

GSSGSSGRPA RQVRRLEFNQ AMDDFKTMFP NMDYDIIECV LRANSGAVDA TIDQLLQMNL ESGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296.0 (±0.1) K, pH 7.0 (±0.05), Details 0.40mM CUE domain U-15N, {13C;} 20mM d-Tris-HCl {(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} {90%H2O;} 10%D2O

#	Name	Isotope labeling	Type	Concentration
1	CUE domain	[U-13C; U-15N]	protein	0.40 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.2 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.2 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DHY, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of the CUE Domain in the Human CUE Domain Containing Protein 1 (CUEDC1)
Zhao, C., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. CUE domain-containing protein 1, : 1 : 2 : 25 entities Detail

Interaction partners 1. CUE domain-containing protein 1, : 5 interactors Detail

Experiments performed 2 experiments Detail