BMRB: 11277

Solution Structure of the RA Domain in the Human Link Guanine Nucleotide Exchange Factor II (Link-GEFII)

Authors

Zhao, C., Kigawa, T., Yoneyama, M., Koshiba, S., Harada, T., Watanabe, S., Yokoyama, S.

Assembly

Rap guanine nucleotide exchange factor (GEF)-like 1

Entity

1. Rap guanine nucleotide exchange factor (GEF)-like 1 (polymer, Thiol state: all free), 120 monomers, 12770.00 Da Detail

GSSGSSGDEI FCRVYMPDHS YVTIRSRLSA SVQDILGSVT EKLQYSEEPA GREDSLILVA VSSSGEKVLL QPTEDCVFTA LGINSHLFAC TRDSYEALVP LPEEIQVSPG DTEISGPSSG

Formula weight

12770.0 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 94.4 %, Completeness (bb): 93.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.4 % (1221 of 1294)	93.2 % (619 of 664)	96.1 % (492 of 512)	93.2 % (110 of 118)
Backbone	93.2 % (658 of 706)	91.8 % (223 of 243)	94.3 % (330 of 350)	92.9 % (105 of 113)
Sidechain	95.6 % (667 of 698)	94.1 % (396 of 421)	97.8 % (266 of 272)	100.0 % (5 of 5)
Aromatic	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)
Methyl	100.0 % (136 of 136)	100.0 % (68 of 68)	100.0 % (68 of 68)

1. RA domain

GSSGSSGDEI FCRVYMPDHS YVTIRSRLSA SVQDILGSVT EKLQYSEEPA GREDSLILVA VSSSGEKVLL QPTEDCVFTA LGINSHLFAC TRDSYEALVP LPEEIQVSPG DTEISGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296.0 (±0.1) K, pH 7.0 (±0.05), Details 1.21mM RA domain U-15N, {13C;} 20mM d-Tris-HCl {(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RA domain	[U-13C; U-15N]	protein	1.21 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.15 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DHZ, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of the RA Domain in the Human Link Guanine Nucleotide Exchange Factor II (Link-GEFII)
Zhao, C., Kigawa, T., Yoneyama, M., Koshiba, S., Harada, T., Watanabe, S., Yokoyama, S.

Related entities 1. Rap guanine nucleotide exchange factor (GEF)-like 1, : 1 : 3 : 37 entities Detail

Interaction partners 1. Rap guanine nucleotide exchange factor (GEF)-like 1, : 2 interactors Detail

Experiments performed 2 experiments Detail