BMRB: 11278

Solution Structure of the CUE Domain in the Human Activating Signal Cointegrator 1 Complex Subunit 2 (ASCC2)

Authors

Zhao, C., Kigawa, T., Tochio, N., Koshiba, S., Harada, T., Watanabe, S., Yokoyama, S.

Assembly

Activating signal cointegrator 1 complex subunit 2

Entity

1. Activating signal cointegrator 1 complex subunit 2 (polymer, Thiol state: all free), 71 monomers, 7886.748 Da Detail

GSSGSSGMCG VELDSLISQV KDLLPDLGEG FILACLEYYH YDPEQVINNI LEERLAPTLS QLDRNLDREM N

Formula weight

7886.748 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.0 %, Completeness: 94.9 %, Completeness (bb): 91.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.9 % (760 of 801)	95.2 % (396 of 416)	95.5 % (296 of 310)	90.7 % (68 of 75)
Backbone	91.9 % (386 of 420)	91.0 % (132 of 145)	93.2 % (193 of 207)	89.7 % (61 of 68)
Sidechain	97.8 % (436 of 446)	97.4 % (264 of 271)	98.2 % (165 of 168)	100.0 % (7 of 7)
Aromatic	100.0 % (38 of 38)	100.0 % (19 of 19)	100.0 % (19 of 19)
Methyl	100.0 % (82 of 82)	100.0 % (41 of 41)	100.0 % (41 of 41)

1. CUE domain

GSSGSSGMCG VELDSLISQV KDLLPDLGEG FILACLEYYH YDPEQVINNI LEERLAPTLS QLDRNLDREM N

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296.0 (±0.1) K, pH 7.0 (±0.05), Details 1.24mM CUE domain U-15N, {13C;} 20mM d-Tris-HCl {(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CUE domain	[U-13C; U-15N]	protein	1.24 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.22 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.22 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DI0, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution Structure of the CUE Domain in the Human Activating Signal Cointegrator 1 Complex Subunit 2 (ASCC2)
Zhao, C., Kigawa, T., Tochio, N., Koshiba, S., Harada, T., Watanabe, S., Yokoyama, S.

Related entities 1. Activating signal cointegrator 1 complex subunit 2, : 1 : 9 entities Detail

Interaction partners 1. Activating signal cointegrator 1 complex subunit 2, : 49 interactors Detail

Experiments performed 2 experiments Detail