Structure of the UBX domain in Mouse UBX Domain-Containing Protein 2
GSSGSSGRDR STIARIQFRL PDGSSFTNQF PSDAPLEEAR QFAAQTVGNT YGNFSLATMF PRREFTREDY KRRLLDLELA PSASVVLLPA GRPATSIVHS SSGDILMID
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 91.1 % (1109 of 1217) | 89.1 % (563 of 632) | 93.9 % (447 of 476) | 90.8 % (99 of 109) |
Backbone | 89.7 % (574 of 640) | 85.8 % (188 of 219) | 92.2 % (294 of 319) | 90.2 % (92 of 102) |
Sidechain | 90.7 % (615 of 678) | 88.6 % (366 of 413) | 93.8 % (242 of 258) | 100.0 % (7 of 7) |
Aromatic | 92.2 % (83 of 90) | 91.1 % (41 of 45) | 93.3 % (42 of 45) | |
Methyl | 94.5 % (104 of 110) | 92.7 % (51 of 55) | 96.4 % (53 of 55) |
1. UBX domain
GSSGSSGRDR STIARIQFRL PDGSSFTNQF PSDAPLEEAR QFAAQTVGNT YGNFSLATMF PRREFTREDY KRRLLDLELA PSASVVLLPA GRPATSIVHS SSGDILMIDSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296.0 (±0.1) K, pH 7.0 (±0.05), Details 1.18mM UBX domain U-15N, {13C;} 20mM d-Tris-HCl (pH7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | UBX domain | [U-13C; U-15N] | protein | 1.18 mM |
2 | d-Tris-HCl | natural abundance | buffer | 20 mM |
3 | NaCl | natural abundance | salt | 100 mM |
4 | d-DTT | natural abundance | salt | 1 mM |
5 | NaN3 | natural abundance | salt | 0.02 % |
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |