BMRB: 11281

Structure of the UBX domain in Mouse UBX Domain-Containing Protein 2

Authors

Zhao, C., Yoneyama, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

UBX domain-containing protein 2

Entity

1. UBX domain-containing protein 2 (polymer, Thiol state: not present), 109 monomers, 11881.10 Da Detail

GSSGSSGRDR STIARIQFRL PDGSSFTNQF PSDAPLEEAR QFAAQTVGNT YGNFSLATMF PRREFTREDY KRRLLDLELA PSASVVLLPA GRPATSIVHS SSGDILMID

Formula weight

11881.1 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 91.1 %, Completeness (bb): 89.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.1 % (1109 of 1217)	89.1 % (563 of 632)	93.9 % (447 of 476)	90.8 % (99 of 109)
Backbone	89.7 % (574 of 640)	85.8 % (188 of 219)	92.2 % (294 of 319)	90.2 % (92 of 102)
Sidechain	90.7 % (615 of 678)	88.6 % (366 of 413)	93.8 % (242 of 258)	100.0 % (7 of 7)
Aromatic	92.2 % (83 of 90)	91.1 % (41 of 45)	93.3 % (42 of 45)
Methyl	94.5 % (104 of 110)	92.7 % (51 of 55)	96.4 % (53 of 55)

1. UBX domain

GSSGSSGRDR STIARIQFRL PDGSSFTNQF PSDAPLEEAR QFAAQTVGNT YGNFSLATMF PRREFTREDY KRRLLDLELA PSASVVLLPA GRPATSIVHS SSGDILMID

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296.0 (±0.1) K, pH 7.0 (±0.05), Details 1.18mM UBX domain U-15N, {13C;} 20mM d-Tris-HCl (pH7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	UBX domain	[U-13C; U-15N]	protein	1.18 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.28 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DZK, Strand ID: A Detail

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Release date

2011-08-17

Citation

Structure of the UBX domain in Mouse UBX Domain-Containing Protein 2
Zhao, C., Yoneyama, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. UBX domain-containing protein 2, : 1 : 10 entities Detail

Interaction partners 1. UBX domain-containing protein 2, : 17 interactors Detail

Experiments performed 2 experiments Detail