BMRB: 11282

Solution Structure of the UBA domain in Human Protein FAM100B

Authors

Zhao, C., Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Protein FAM100B

Entity

1. Protein FAM100B (polymer, Thiol state: all free), 66 monomers, 7263.963 Da Detail

GSSGSSGMSV NMDELRHQVM INQFVLAAGC AADQAKQLLQ AAHWQFETAL STFFQETNIP NSHHHH

Formula weight

7263.963 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.8 %, Completeness: 81.5 %, Completeness (bb): 79.2 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	81.5 % (603 of 740)	80.5 % (305 of 379)	82.7 % (235 of 284)	81.8 % (63 of 77)
Backbone	79.2 % (312 of 394)	76.3 % (103 of 135)	81.4 % (158 of 194)	78.5 % (51 of 65)
Sidechain	84.1 % (343 of 408)	82.8 % (202 of 244)	84.9 % (129 of 152)	100.0 % (12 of 12)
Aromatic	73.7 % (56 of 76)	73.7 % (28 of 38)	73.0 % (27 of 37)	100.0 % (1 of 1)
Methyl	96.8 % (60 of 62)	96.8 % (30 of 31)	96.8 % (30 of 31)

1. UBA-like domain, residues 8-66

GSSGSSGMSV NMDELRHQVM INQFVLAAGC AADQAKQLLQ AAHWQFETAL STFFQETNIP NSHHHH

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296.0 (±0.1) K, pH 7.0 (±0.05), Details 0.43mM UBA-like domain U-15N, {13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O, 90% H2O

#	Name	Isotope labeling	Type	Concentration
1	UBA-like domain	[U-13C; U-15N]	protein	0.43 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.08 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.2 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2DZL, Strand ID: A Detail

---

Release date

2011-08-17

Citation

Solution Structure of the UBA domain in Human Protein FAM100B
Zhao, C., Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Protein FAM100B, : 1 : 2 : 22 entities Detail

Interaction partners 1. Protein FAM100B, : 2 interactors Detail

Experiments performed 2 experiments Detail