BMRB: 11283

Solution Structure of the Ubiquitin-like Domain in Human FAS-associated factor 1 (hFAF1)

Authors

Zhao, C., Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

FAS-associated factor 1

Entity

1. FAS-associated factor 1 (polymer, Thiol state: all free), 100 monomers, 10953.23 Da Detail

GSSGSSGRML DFRVEYRDRN VDVVLEDTCT VGEIKQILEN ELQIPVSKML LKGWKTGDVE DSTVLKSLHL PKNNSLYVLT PDLPPPSSSS HAGALQESLN

Formula weight

10953.23 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.0 %, Completeness: 90.9 %, Completeness (bb): 90.8 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.9 % (1031 of 1134)	89.8 % (530 of 590)	92.5 % (408 of 441)	90.3 % (93 of 103)
Backbone	90.8 % (534 of 588)	90.0 % (181 of 201)	91.1 % (267 of 293)	91.5 % (86 of 94)
Sidechain	91.1 % (582 of 639)	89.7 % (349 of 389)	93.8 % (226 of 241)	77.8 % (7 of 9)
Aromatic	87.0 % (40 of 46)	87.0 % (20 of 23)	86.4 % (19 of 22)	100.0 % (1 of 1)
Methyl	99.2 % (117 of 118)	98.3 % (58 of 59)	100.0 % (59 of 59)

1. ubiquitin-like domain, residues 8-100

GSSGSSGRML DFRVEYRDRN VDVVLEDTCT VGEIKQILEN ELQIPVSKML LKGWKTGDVE DSTVLKSLHL PKNNSLYVLT PDLPPPSSSS HAGALQESLN

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296.0 (±0.1) K, pH 7.0 (±0.05), Details 1.17mM ubiquitin-like domain U-15N, {13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O, 90% H2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like domain	[U-13C; U-15N]	protein	1.17 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.24 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2DZM, Strand ID: A Detail

---

Release date

2011-08-17

Citation

Solution Structure of the Ubiquitin-like Domain in Human FAS-associated factor 1 (hFAF1)
Zhao, C., Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. FAS-associated factor 1, : 1 : 2 : 7 entities Detail

Interaction partners 1. FAS-associated factor 1, : 38 interactors Detail

Experiments performed 2 experiments Detail