BMRB: 11284

Solution structure of the SH3 domain of human hypothetical protein FLJ21522

Authors

Yoneyama, M., Saito, K., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Hypothetical protein FLJ21522

Entity

1. Hypothetical protein FLJ21522 (polymer, Thiol state: not present), 68 monomers, 7473.310 Da Detail

GSSGSSGLKM QVLYEFEARN PRELTVVQGE KLEVLDHSKR WWLVKNEAGR SGYIPSNILE PLSGPSSG

Formula weight

7473.31 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.2 %, Completeness: 91.2 %, Completeness (bb): 86.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.2 % (714 of 783)	91.2 % (375 of 411)	92.7 % (279 of 301)	84.5 % (60 of 71)
Backbone	86.3 % (345 of 400)	84.3 % (118 of 140)	88.8 % (174 of 196)	82.8 % (53 of 64)
Sidechain	95.7 % (424 of 443)	94.8 % (257 of 271)	97.0 % (160 of 165)	100.0 % (7 of 7)
Aromatic	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (25 of 25)	100.0 % (2 of 2)
Methyl	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)

1. SH3 domain

GSSGSSGLKM QVLYEFEARN PRELTVVQGE KLEVLDHSKR WWLVKNEAGR SGYIPSNILE PLSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.33mM SH3 domain U-15N, {13C;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10%D2O

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.33 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.36 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WXT, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution structure of the SH3 domain of human hypothetical protein FLJ21522
Yoneyama, M., Saito, K., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Hypothetical protein FLJ21522, : 1 : 2 : 135 entities Detail

Interaction partners 1. Hypothetical protein FLJ21522, : 4 interactors Detail

Experiments performed 2 experiments Detail