BMRB: 11285

Solution structure of the SH3 domain of mouse peroxisomal biogenesis factor 13

Authors

Yoneyama, M., Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

peroxisomal biogenesis factor 13

Entity

1. peroxisomal biogenesis factor 13 (polymer, Thiol state: not present), 93 monomers, 9908.874 Da Detail

GSSGSSGTNW ASGEDDHVVA RAEYDFVAVS DEEISFRAGD MLNLALKEQQ PKVRGWLLAS LDGQTTGLIP ANYVKILGKR RGRKTIESGP SSG

Formula weight

9908.874 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.5 %, Completeness: 90.4 %, Completeness (bb): 86.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.4 % (921 of 1019)	91.1 % (481 of 528)	89.3 % (351 of 393)	90.8 % (89 of 98)
Backbone	86.4 % (477 of 552)	87.7 % (171 of 195)	84.3 % (225 of 267)	90.0 % (81 of 90)
Sidechain	94.9 % (520 of 548)	93.1 % (310 of 333)	97.6 % (202 of 207)	100.0 % (8 of 8)
Aromatic	90.6 % (58 of 64)	81.3 % (26 of 32)	100.0 % (30 of 30)	100.0 % (2 of 2)
Methyl	100.0 % (96 of 96)	100.0 % (48 of 48)	100.0 % (48 of 48)

1. SH3 domain

GSSGSSGTNW ASGEDDHVVA RAEYDFVAVS DEEISFRAGD MLNLALKEQQ PKVRGWLLAS LDGQTTGLIP ANYVKILGKR RGRKTIESGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.09mM SH3 domain U-15N, {13C;} 20mM {d-Tris-HCl;} 200mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10%D2O

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.09 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	200 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.2 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.2 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.13 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WXU, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution structure of the SH3 domain of mouse peroxisomal biogenesis factor 13
Yoneyama, M., Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. peroxisomal biogenesis factor 13, : 1 : 63 entities Detail

Interaction partners 1. peroxisomal biogenesis factor 13, : 6 interactors Detail

Experiments performed 2 experiments Detail