BMRB: 11287

Solution structure of the C2H2 type zinc-binding domain of human zinc finger protein 292

Authors

Yoneyama, M., Tomizawa, T., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 292

Entity

1. C2H2 type zinc-binding domain (polymer, Thiol state: all other bound), 73 monomers, 8004.764 Da Detail

GSSGSSGRKK PVSQSLEFPT RYSPYRPYRC VHQGCFAAFT IQQNLILHYQ AVHKSDLPAF SAEVEEESGP SSG

2. ZN (non-polymer), 65.409 Da

Total weight

8070.1733 Da

Max. entity weight

8004.764 Da

Entity Connection

na 4 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS30:SG	2:ZN1:ZN
2	na	sing	1:CYS35:SG	2:ZN1:ZN
3	na	sing	1:HIS48:NE2	2:ZN1:ZN
4	na	sing	1:HIS53:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.2 %, Completeness: 92.0 %, Completeness (bb): 90.6 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.0 % (762 of 828)	92.4 % (400 of 433)	92.9 % (299 of 322)	86.3 % (63 of 73)
Backbone	90.6 % (386 of 426)	89.7 % (131 of 146)	92.0 % (196 of 213)	88.1 % (59 of 67)
Sidechain	93.8 % (440 of 469)	93.7 % (269 of 287)	94.9 % (167 of 176)	66.7 % (4 of 6)
Aromatic	85.7 % (72 of 84)	85.7 % (36 of 42)	85.7 % (36 of 42)
Methyl	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (27 of 27)

1. C2H2 type zinc-binding domain

GSSGSSGRKK PVSQSLEFPT RYSPYRPYRC VHQGCFAAFT IQQNLILHYQ AVHKSDLPAF SAEVEEESGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.90mM C2H2 type zinc-binding domain U-15N, {13C;} 20mM d-Tris-HCl {(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM {ZnCl2;} 0.1mM {NTA;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	C2H2 type zinc-binding domain	[U-13C; U-15N]	protein	1.90 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	NTA	natural abundance	salt	0.1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.19 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1X3C, Strand ID: A Detail

---

Release date

2011-08-17

Citation

Solution structure of the C2H2 type zinc-binding domain of human zinc finger protein 292
Yoneyama, M., Tomizawa, T., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. C2H2 type zinc-binding domain, : 1 : 1 : 5 entities Detail

Interaction partners 1. C2H2 type zinc-binding domain, : 14 interactors Detail

Experiments performed 2 experiments Detail