BMRB: 11288

Solution structure of the fibronectin type-III domain of human fibronectin type-III domain containing protein 3a

Authors

Yoneyama, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Fibronectin type-III domain containing protein 3a

Entity

1. Fibronectin type-III domain containing protein 3a (polymer, Thiol state: all free), 118 monomers, 12736.02 Da Detail

GSSGSSGAEI FTTLSCEPDI PNPPRIANRT KNSLTLQWKA PSDNGSKIQN FVLEWDEGKG NGEFCQCYMG SQKQFKITKL SPAMGCKFRL SARNDYGTSG FSEEVLYYTS GCSGPSSG

Formula weight

12736.02 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.2 %, Completeness: 93.0 %, Completeness (bb): 91.5 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.0 % (1230 of 1322)	92.3 % (639 of 692)	93.9 % (474 of 505)	93.6 % (117 of 125)
Backbone	91.5 % (635 of 694)	89.7 % (218 of 243)	92.4 % (314 of 340)	92.8 % (103 of 111)
Sidechain	94.5 % (692 of 732)	93.8 % (421 of 449)	95.5 % (257 of 269)	100.0 % (14 of 14)
Aromatic	92.2 % (107 of 116)	89.7 % (52 of 58)	94.6 % (53 of 56)	100.0 % (2 of 2)
Methyl	100.0 % (80 of 80)	100.0 % (40 of 40)	100.0 % (40 of 40)

1. Fibronectin type-III domain

GSSGSSGAEI FTTLSCEPDI PNPPRIANRT KNSLTLQWKA PSDNGSKIQN FVLEWDEGKG NGEFCQCYMG SQKQFKITKL SPAMGCKFRL SARNDYGTSG FSEEVLYYTS GCSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.16mM fn3 domain U-15N, {13C;} 20mM d-Tris-HCl {(pH7.0);} 100mM {NaCl;} 5mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	fn3 domain	[U-13C; U-15N]	protein	1.16 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	5 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.92 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1X3D, Strand ID: A Detail

---

Release date

2011-08-17

Citation

Solution structure of the fibronectin type-III domain of human fibronectin type-III domain containing protein 3a
Yoneyama, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Fibronectin type-III domain containing protein 3a, : 1 : 268 entities Detail

Interaction partners 1. Fibronectin type-III domain containing protein 3a, : 27 interactors Detail

Experiments performed 2 experiments Detail