BMRB: 11292

Solution structure of the fibronectin type-III domain of human protein tyrosine phosphatase, receptor type, D isoform 4 variant

Authors

Yoneyama, M., Saito, K., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Receptor-type tyrosine-protein phosphatase delta

Entity

1. Receptor-type tyrosine-protein phosphatase delta (polymer, Thiol state: not present), 115 monomers, 12262.32 Da Detail

GSSGSSGDIQ VITQTGVPGQ PLNFKAEPES ETSILLSWTP PRSDTIANYE LVYKDGEHGE EQRITIEPGT SYRLQGLKPN SLYYFRLAAR SPQGLGASTA EISARTMQSS GPSSG

Formula weight

12262.32 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.0 %, Completeness: 91.6 %, Completeness (bb): 89.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.6 % (1159 of 1265)	91.4 % (602 of 659)	92.2 % (451 of 489)	90.6 % (106 of 117)
Backbone	89.1 % (599 of 672)	88.0 % (206 of 234)	89.5 % (297 of 332)	90.6 % (96 of 106)
Sidechain	94.7 % (658 of 695)	93.2 % (396 of 425)	97.3 % (252 of 259)	90.9 % (10 of 11)
Aromatic	97.4 % (74 of 76)	97.4 % (37 of 38)	97.3 % (36 of 37)	100.0 % (1 of 1)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. Fibronectin type-III domain

GSSGSSGDIQ VITQTGVPGQ PLNFKAEPES ETSILLSWTP PRSDTIANYE LVYKDGEHGE EQRITIEPGT SYRLQGLKPN SLYYFRLAAR SPQGLGASTA EISARTMQSS GPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.37mM fn3 domain U-15N, {13C;} 20mM d-Tris-HCl {(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	fn3 domain	[U-13C; U-15N]	protein	1.37 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.51 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X5Z, Strand ID: A Detail

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Release date

2011-08-17

Citation

Solution structure of the fibronectin type-III domain of human protein tyrosine phosphatase, receptor type, D isoform 4 variant
Yoneyama, M., Saito, K., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Receptor-type tyrosine-protein phosphatase delta, : 1 : 2 : 377 entities Detail

Interaction partners 1. Receptor-type tyrosine-protein phosphatase delta, : 5 interactors Detail

Experiments performed 2 experiments Detail