BMRB: 11294

Solution structure of the RWD domain of human RWD omain containing protein 2

Authors

Yoneyama, M., Kigawa, T., Sato, M., Tochio, N., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

RWD domain containing protein 2

Entity

1. RWD domain containing protein 2 (polymer, Thiol state: all free), 154 monomers, 17113.44 Da Detail

GSSGSSGMSA SVKESLQLQL LEMEMLFSMF PNQGEVKLED VNALTNIKRY LEGTREALPP KIEFVITLQI EEPKVKIDLQ VTMPHSYPYL ALQLFGRSSE LDRHQQLLLN KGLTSYIGTF DPGELCVCAA IQWLQDNSAS YFLNRKLVSG PSSG

Formula weight

17113.44 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.8 %, Completeness: 93.2 %, Completeness (bb): 90.2 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.2 % (1688 of 1811)	94.4 % (891 of 944)	91.9 % (647 of 704)	92.0 % (150 of 163)
Backbone	90.2 % (819 of 908)	92.3 % (287 of 311)	88.2 % (398 of 451)	91.8 % (134 of 146)
Sidechain	96.1 % (1005 of 1046)	95.4 % (604 of 633)	97.2 % (385 of 396)	94.1 % (16 of 17)
Aromatic	100.0 % (120 of 120)	100.0 % (60 of 60)	100.0 % (59 of 59)	100.0 % (1 of 1)
Methyl	100.0 % (178 of 178)	100.0 % (89 of 89)	100.0 % (89 of 89)

1. RWD domain

GSSGSSGMSA SVKESLQLQL LEMEMLFSMF PNQGEVKLED VNALTNIKRY LEGTREALPP KIEFVITLQI EEPKVKIDLQ VTMPHSYPYL ALQLFGRSSE LDRHQQLLLN KGLTSYIGTF DPGELCVCAA IQWLQDNSAS YFLNRKLVSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.17mM RWD domain {U-15N,13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RWD domain	[U-13C; U-15N]	protein	1.17 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2DAW, Strand ID: A Detail

---

Release date

2011-08-18

Citation

Solution structure of the RWD domain of human RWD omain containing protein 2
Yoneyama, M., Kigawa, T., Sato, M., Tochio, N., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. RWD domain containing protein 2, : 1 : 15 entities Detail

Experiments performed 2 experiments Detail