BMRB: 11295

Solution structure of the RWD domain of human protein C21orf6

Authors

Yoneyama, M., Kigawa, T., Saito, K., Tochio, N., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Protein C21orf6

Entity

1. Protein C21orf6 (polymer, Thiol state: all free), 152 monomers, 16548.51 Da Detail

GSSGSSGEQA EAQLAELDLL ASMFPGENEL IVNDQLAVAE LKDCIEKKTM EGRSSKVYFT INMNLDVSDE KMAMFSLACI LPFKYPAVLP EITVRSVLLS RSQQTQLNTD LTAFLQKHCH GDVCILNATE WVREHASGYV SRDTSSSGPS SG

Formula weight

16548.51 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 94.3 %, Completeness (bb): 92.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.3 % (1602 of 1699)	94.5 % (828 of 876)	94.4 % (625 of 662)	92.5 % (149 of 161)
Backbone	92.2 % (832 of 902)	91.9 % (283 of 308)	92.6 % (414 of 447)	91.8 % (135 of 147)
Sidechain	96.6 % (908 of 940)	96.0 % (545 of 568)	97.5 % (349 of 358)	100.0 % (14 of 14)
Aromatic	91.8 % (90 of 98)	91.8 % (45 of 49)	91.7 % (44 of 48)	100.0 % (1 of 1)
Methyl	100.0 % (172 of 172)	100.0 % (86 of 86)	100.0 % (86 of 86)

1. RWD domain

GSSGSSGEQA EAQLAELDLL ASMFPGENEL IVNDQLAVAE LKDCIEKKTM EGRSSKVYFT INMNLDVSDE KMAMFSLACI LPFKYPAVLP EITVRSVLLS RSQQTQLNTD LTAFLQKHCH GDVCILNATE WVREHASGYV SRDTSSSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.26mM RWD domain {U-15N,13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RWD domain	[U-13C; U-15N]	protein	1.26 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DAX, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the RWD domain of human protein C21orf6
Yoneyama, M., Kigawa, T., Saito, K., Tochio, N., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Protein C21orf6, : 1 : 14 entities Detail

Interaction partners 1. Protein C21orf6, : 11 interactors Detail

Experiments performed 2 experiments Detail