BMRB: 11296

Solution structure of the RWD domain of human ring finger protein 25

Authors

Yoneyama, M., Kigawa, T., Sato, M., Tochio, N., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

RING finger protein 25

Entity

1. RING finger protein 25 (polymer, Thiol state: all free), 128 monomers, 13959.48 Da Detail

GSSGSSGEED WVLPSEVEVL ESIYLDELQV IKGNGRTSPW EIYITLHPAT AEDQDSQYVC FTLVLQVPAE YPHEVPQISI RNPRGLSDEQ IHTILQVLGH VAKAGLGTAM LYELIEKGKE ILSGPSSG

Formula weight

13959.48 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 95.2 %, Completeness (bb): 93.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.2 % (1378 of 1448)	95.3 % (712 of 747)	95.3 % (543 of 570)	93.9 % (123 of 131)
Backbone	93.8 % (705 of 752)	93.8 % (244 of 260)	93.8 % (349 of 372)	93.3 % (112 of 120)
Sidechain	96.9 % (787 of 812)	96.1 % (468 of 487)	98.1 % (308 of 314)	100.0 % (11 of 11)
Aromatic	91.1 % (82 of 90)	91.1 % (41 of 45)	90.7 % (39 of 43)	100.0 % (2 of 2)
Methyl	100.0 % (160 of 160)	100.0 % (80 of 80)	100.0 % (80 of 80)

1. RWD domain

GSSGSSGEED WVLPSEVEVL ESIYLDELQV IKGNGRTSPW EIYITLHPAT AEDQDSQYVC FTLVLQVPAE YPHEVPQISI RNPRGLSDEQ IHTILQVLGH VAKAGLGTAM LYELIEKGKE ILSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 0.72mM RWD domain {U-15N,13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RWD domain	[U-13C; U-15N]	protein	0.72 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.22 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DAY, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the RWD domain of human ring finger protein 25
Yoneyama, M., Kigawa, T., Sato, M., Tochio, N., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. RING finger protein 25, : 1 : 67 entities Detail

Interaction partners 1. RING finger protein 25, : 11 interactors Detail

Experiments performed 2 experiments Detail