BMRB: 11297

Solusion structure of the Todor domain of human Lamin-B receptor

Authors

Yoneyama, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Lamin-B receptor

Entity

1. Lamin-B receptor (polymer, Thiol state: not present), 68 monomers, 7358.945 Da Detail

GSSGSSGMPS RKFADGEVVR GRWPGSSLYY EVEILSHDST SQLYTVKYKD GTELELKEND IKSGPSSG

Formula weight

7358.945 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.8 %, Completeness: 88.4 %, Completeness (bb): 83.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.4 % (663 of 750)	88.7 % (347 of 391)	89.0 % (259 of 291)	83.8 % (57 of 68)
Backbone	83.3 % (335 of 402)	82.4 % (117 of 142)	84.1 % (164 of 195)	83.1 % (54 of 65)
Sidechain	93.6 % (381 of 407)	92.4 % (230 of 249)	95.5 % (148 of 155)	100.0 % (3 of 3)
Aromatic	96.6 % (56 of 58)	96.6 % (28 of 29)	96.4 % (27 of 28)	100.0 % (1 of 1)
Methyl	96.2 % (50 of 52)	92.3 % (24 of 26)	100.0 % (26 of 26)

1. Tudor domain

GSSGSSGMPS RKFADGEVVR GRWPGSSLYY EVEILSHDST SQLYTVKYKD GTELELKEND IKSGPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.17mM Tudor domain {U-15N,13C;} 20mM d-Tris HCl (pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tudor domain	[U-13C; U-15N]	protein	1.17 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.21 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.21 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.38 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DIG, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solusion structure of the Todor domain of human Lamin-B receptor
Yoneyama, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Lamin-B receptor, : 1 : 2 : 64 entities Detail

Interaction partners 1. Lamin-B receptor, : 33 interactors Detail

Experiments performed 2 experiments Detail