BMRB: 11298

Solution structure of the BSD domain of human TFIIH basal transcription factor complex p62 subunit

Authors

Yoneyama, M., Tochio, N., Koshiba, S., Watabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

TFIIH basal transcription factor complex p62 subunit

Entity

1. TFIIH basal transcription factor complex p62 subunit (polymer, Thiol state: not present), 61 monomers, 6773.468 Da Detail

GSSGSSGFKR KANKELEEKN RMLQEDPVLF QLYKDLVVSQ VISAEEFWAN RLNVNSGPSS G

Formula weight

6773.468 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.9 %, Completeness: 87.9 %, Completeness (bb): 84.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.9 % (626 of 712)	87.2 % (327 of 375)	89.6 % (241 of 269)	85.3 % (58 of 68)
Backbone	84.3 % (305 of 362)	80.8 % (101 of 125)	86.5 % (154 of 178)	84.7 % (50 of 59)
Sidechain	91.4 % (371 of 406)	90.4 % (226 of 250)	93.2 % (137 of 147)	88.9 % (8 of 9)
Aromatic	84.0 % (42 of 50)	84.0 % (21 of 25)	83.3 % (20 of 24)	100.0 % (1 of 1)
Methyl	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (27 of 27)

1. BSD domain

GSSGSSGFKR KANKELEEKN RMLQEDPVLF QLYKDLVVSQ VISAEEFWAN RLNVNSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.07mM BSD domain {U-15N,13C;} 20mM d-Tris HCl (pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	BSD domain	[U-13C; U-15N]	protein	1.07 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.81 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.2 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DII, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the BSD domain of human TFIIH basal transcription factor complex p62 subunit
Yoneyama, M., Tochio, N., Koshiba, S., Watabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. TFIIH basal transcription factor complex p62 subunit, : 1 : 36 entities Detail

Interaction partners 1. TFIIH basal transcription factor complex p62 subunit, : 1 interactors Detail

Experiments performed 2 experiments Detail