BMRB: 11300

Solution structure of the Myb_DNA-binding domain of human Cell division cycle 5-like protein

Authors

Yoneyama, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Cell division cycle 5-like protein

Entity

1. Cell division cycle 5-like protein (polymer, Thiol state: all free), 70 monomers, 7812.731 Da Detail

GSSGSSGKGG VWRNTEDEIL KAAVMKYGKN QWSRIASLLH RKSAKQCKAR WYEWLDPSIK KTEWSGPSSG

Formula weight

7812.731 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.4 %, Completeness: 90.7 %, Completeness (bb): 86.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.7 % (745 of 821)	91.7 % (397 of 433)	90.4 % (281 of 311)	87.0 % (67 of 77)
Backbone	86.1 % (358 of 416)	86.3 % (126 of 146)	86.1 % (174 of 202)	85.3 % (58 of 68)
Sidechain	95.3 % (445 of 467)	94.4 % (271 of 287)	96.5 % (165 of 171)	100.0 % (9 of 9)
Aromatic	95.0 % (76 of 80)	95.0 % (38 of 40)	94.3 % (33 of 35)	100.0 % (5 of 5)
Methyl	98.0 % (49 of 50)	96.0 % (24 of 25)	100.0 % (25 of 25)

1. Myb DNA-binding domain

GSSGSSGKGG VWRNTEDEIL KAAVMKYGKN QWSRIASLLH RKSAKQCKAR WYEWLDPSIK KTEWSGPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.19mM Myb_DNA-binding domain U-15N, {13C;} 20mM d-Tris HCl (pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Myb_DNA-binding domain	[U-13C; U-15N]	protein	1.19 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.29 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DIM, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the Myb_DNA-binding domain of human Cell division cycle 5-like protein
Yoneyama, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Cell division cycle 5-like protein, : 1 : 32 : 215 entities Detail

Interaction partners 1. Cell division cycle 5-like protein, : 12 interactors Detail

Experiments performed 2 experiments Detail