Solution structure of the Myb_DNA-binding domain of human Cell division cycle 5-like protein
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 87.5 % (659 of 753) | 87.1 % (344 of 395) | 88.3 % (256 of 290) | 86.8 % (59 of 68) |
Backbone | 83.1 % (324 of 390) | 81.5 % (110 of 135) | 83.3 % (160 of 192) | 85.7 % (54 of 63) |
Sidechain | 91.7 % (388 of 423) | 90.0 % (234 of 260) | 94.3 % (149 of 158) | 100.0 % (5 of 5) |
Aromatic | 100.0 % (50 of 50) | 100.0 % (25 of 25) | 100.0 % (23 of 23) | 100.0 % (2 of 2) |
Methyl | 100.0 % (60 of 60) | 100.0 % (30 of 30) | 100.0 % (30 of 30) |
1. Myb DNA-binding domain
GSSGSSGKKT EWSREEEEKL LHLAKLMPTQ WRTIAPIIGR TAAQCLEHYE FLLDKAAQRD SGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.13mM Myb_DNA-binding domain U-15N, {13C;} 20mM d-Tris HCl (pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Myb_DNA-binding domain | [U-13C; U-15N] | protein | 1.13 mM |
2 | d-Tris-HCl | natural abundance | buffer | 20 mM |
3 | NaCl | natural abundance | salt | 100 mM |
4 | d-DTT | natural abundance | salt | 1 mM |
5 | NaN3 | natural abundance | salt | 0.02 % |
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |