BMRB: 11302

Solution structure of the N-terminal C2H2 type zinc-binding domain of the Zinc finger protein 64, isoforms 1 and 2

Authors

Yoneyama, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 64, isoforms 1 and 2

Entity

1. C2H2-type zinc-binding domain (polymer, Thiol state: free and other bound), 96 monomers, 10469.71 Da Detail

GSSGSSGPHK CEVCGKCFSR KDKLKTHMRC HTGVKPYKCK TCDYAAADSS SLNKHLRIHS DERPFKCQIC PYASRNSSQL TVHLRSHTGD SGPSSG

2. ZN (non-polymer), 65.409 × 3 Da

Total weight

10665.937 Da

Max. entity weight

10469.71 Da

Entity Connection

na 12 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS11:SG	2:ZN1:ZN
2	na	sing	1:CYS14:SG	2:ZN1:ZN
3	na	sing	1:HIS27:NE2	2:ZN1:ZN
4	na	sing	1:HIS31:NE2	2:ZN1:ZN
5	na	sing	1:CYS39:SG	2:ZN1:ZN
6	na	sing	1:CYS42:SG	2:ZN1:ZN
7	na	sing	1:HIS55:NE2	2:ZN1:ZN
8	na	sing	1:HIS59:NE2	2:ZN1:ZN
9	na	sing	1:CYS67:SG	2:ZN1:ZN
10	na	sing	1:CYS70:SG	2:ZN1:ZN
11	na	sing	1:HIS83:NE2	2:ZN1:ZN
12	na	sing	1:HIS87:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.8 %, Completeness: 90.6 %, Completeness (bb): 88.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.6 % (958 of 1057)	90.5 % (503 of 556)	92.4 % (375 of 406)	84.2 % (80 of 95)
Backbone	88.3 % (500 of 566)	86.7 % (169 of 195)	90.4 % (253 of 280)	85.7 % (78 of 91)
Sidechain	93.6 % (542 of 579)	92.5 % (334 of 361)	96.3 % (206 of 214)	50.0 % (2 of 4)
Aromatic	86.1 % (62 of 72)	83.3 % (30 of 36)	88.9 % (32 of 36)
Methyl	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (29 of 29)

1. C2H2-type zinc-binding domain

GSSGSSGPHK CEVCGKCFSR KDKLKTHMRC HTGVKPYKCK TCDYAAADSS SLNKHLRIHS DERPFKCQIC PYASRNSSQL TVHLRSHTGD SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.28mM C2H2 type zinc-binding domain U-15N, {13C;} 20mM d-Tris {HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 50uM ZnCl2 + 1mM {IDA;} 10% D2O, 90% H2O

#	Name	Isotope labeling	Type	Concentration
1	C2H2 type zinc-binding domain	[U-13C; U-15N]	protein	1.28 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	50 uM
7	IDA	natural abundance	salt	1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.2 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.2 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DMD, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the N-terminal C2H2 type zinc-binding domain of the Zinc finger protein 64, isoforms 1 and 2
Yoneyama, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. C2H2-type zinc-binding domain, : 1 : 4 : 2149 entities Detail

Interaction partners 1. C2H2-type zinc-binding domain, : 15 interactors Detail

Experiments performed 2 experiments Detail