BMRB: 11303

An extended conformation of the RWD domain of human Ring finger protein 25

Authors

Yoneyama, M., Tochio, N., Koshiba, S., Watabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

RING finger protein 25

Entity

1. RING finger protein 25 (polymer, Thiol state: all free), 137 monomers, 14936.47 Da Detail

GSSGSSGEED WVLPSEVEVL ESIYLDELQV IKGNGRTSPW EIYITLHPAT AEDQDSQYVC FTLVLQVPAE YPHEVPQISI RNPRGLSDEQ IHTILQVLGH VAKAGLGTAM LYELIEKGKE ILTDNNIPHG QSGPSSG

Formula weight

14936.47 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.2 %, Completeness: 93.2 %, Completeness (bb): 92.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.2 % (1440 of 1545)	92.6 % (739 of 798)	93.9 % (568 of 605)	93.7 % (133 of 142)
Backbone	92.4 % (743 of 804)	91.7 % (255 of 278)	92.2 % (367 of 398)	94.5 % (121 of 128)
Sidechain	94.1 % (814 of 865)	93.1 % (484 of 520)	96.1 % (318 of 331)	85.7 % (12 of 14)
Aromatic	93.6 % (88 of 94)	93.6 % (44 of 47)	93.3 % (42 of 45)	100.0 % (2 of 2)
Methyl	98.2 % (163 of 166)	97.6 % (81 of 83)	98.8 % (82 of 83)

1. RWD domain

GSSGSSGEED WVLPSEVEVL ESIYLDELQV IKGNGRTSPW EIYITLHPAT AEDQDSQYVC FTLVLQVPAE YPHEVPQISI RNPRGLSDEQ IHTILQVLGH VAKAGLGTAM LYELIEKGKE ILTDNNIPHG QSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 0.94mM RWD domain U-15N, {13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O, 90% H2O

#	Name	Isotope labeling	Type	Concentration
1	RWD domain	[U-13C; U-15N]	protein	0.94 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.24 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DMF, Strand ID: A Detail

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Release date

2011-08-18

Citation

An extended conformation of the RWD domain of human Ring finger protein 25
Yoneyama, M., Tochio, N., Koshiba, S., Watabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. RING finger protein 25, : 1 : 61 entities Detail

Interaction partners 1. RING finger protein 25, : 11 interactors Detail

Experiments performed 2 experiments Detail