BMRB: 11305

Solution Structure of the LIM Domain of the Human Actin Binding LIM Protein 2

Authors

Miyamoto, K., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Actin Binding LIM Protein 2

Entity

1. LIM Domain (polymer, Thiol state: free and other bound), 81 monomers, 8621.709 Da Detail

GSSGSSGLDY QRLYGTRCFS CDQFIEGEVV SALGKTYHPD CFVCAVCRLP FPPGDRVTFN GKECMCQKCS LPVSVSGPSS G

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

8752.527 Da

Max. entity weight

8621.709 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:HIS38:ND1	2:ZN1:ZN
4	na	sing	1:CYS41:SG	2:ZN1:ZN
5	na	sing	1:CYS44:SG	2:ZN1:ZN
6	na	sing	1:CYS47:SG	2:ZN1:ZN
7	na	sing	1:CYS66:SG	2:ZN1:ZN
8	na	sing	1:CYS69:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.4 %, Completeness: 90.8 %, Completeness (bb): 88.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.8 % (797 of 878)	90.5 % (412 of 455)	91.9 % (316 of 344)	87.3 % (69 of 79)
Backbone	88.0 % (417 of 474)	87.3 % (145 of 166)	88.8 % (207 of 233)	86.7 % (65 of 75)
Sidechain	93.7 % (445 of 475)	92.4 % (267 of 289)	95.6 % (174 of 182)	100.0 % (4 of 4)
Aromatic	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (39 of 39)
Methyl	100.0 % (62 of 62)	100.0 % (31 of 31)	100.0 % (31 of 31)

1. LIM Domain

GSSGSSGLDY QRLYGTRCFS CDQFIEGEVV SALGKTYHPD CFVCAVCRLP FPPGDRVTFN GKECMCQKCS LPVSVSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.5 (±0.05), Details 1.22mM LIM Domain {U-13C,15N;} 20mM d-Tris-HCl(pH {7.5);} 100mM {NaCl;} 0.02% {NaN3;} 0.1mM {ZnCl2;} 90% H2O,10% D2O

#	Name	Isotope labeling	Type	Concentration
1	LIM Domain	[U-13C; U-15N]	protein	1.22 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	NaN3	natural abundance	salt	0.02 %
5	ZnCl2	natural abundance	salt	0.1 mM
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V6G, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution Structure of the LIM Domain of the Human Actin Binding LIM Protein 2
Miyamoto, K., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. LIM Domain, : 1 : 2 : 72 entities Detail

Interaction partners 1. LIM Domain, : 5 interactors Detail

Experiments performed 2 experiments Detail