BMRB: 11306

Solution Structure of the Ring-H2 Finger Domain of Mouse Deltex Protein 2

Authors

Miyamoto, K., Muto, Y., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Deltex protein 2

Entity

1. Ring-H2 Finger Domain (polymer, Thiol state: free and other bound), 114 monomers, 12215.89 Da Detail

GSSGSSGEPE QVIRKYTEEL KVAPEEDCII CMEKLAVASG YSDMTDSKAL GPMVVGRLTK CSHAFHLLCL LAMYCNGNKD GSLQCPSCKT IYGEKTGTQP WGKMEVFRSG PSSG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

12346.708 Da

Max. entity weight

12215.89 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS28:SG	2:ZN1:ZN
2	na	sing	1:CYS31:SG	2:ZN1:ZN
3	na	sing	1:HIS66:ND1	2:ZN1:ZN
4	na	sing	1:CYS69:SG	2:ZN1:ZN
5	na	sing	1:CYS61:SG	2:ZN1:ZN
6	na	sing	1:HIS63:ND1	2:ZN1:ZN
7	na	sing	1:CYS85:SG	2:ZN1:ZN
8	na	sing	1:CYS88:SG	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

Torsion Angle Dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 96.3 %, Completeness (bb): 94.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.3 % (1210 of 1256)	96.3 % (630 of 654)	96.5 % (471 of 488)	95.6 % (109 of 114)
Backbone	94.8 % (637 of 672)	94.5 % (222 of 235)	94.8 % (312 of 329)	95.4 % (103 of 108)
Sidechain	97.7 % (669 of 685)	97.4 % (408 of 419)	98.1 % (255 of 260)	100.0 % (6 of 6)
Aromatic	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (35 of 35)	100.0 % (1 of 1)
Methyl	100.0 % (100 of 100)	100.0 % (50 of 50)	100.0 % (50 of 50)

1. Ring-H2 Finger Domain

GSSGSSGEPE QVIRKYTEEL KVAPEEDCII CMEKLAVASG YSDMTDSKAL GPMVVGRLTK CSHAFHLLCL LAMYCNGNKD GSLQCPSCKT IYGEKTGTQP WGKMEVFRSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.05mM Ring-H2 Domain U-13C, {15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.1mM {ZnCl;} 90% H2O, 10%D2O

#	Name	Isotope labeling	Type	Concentration
1	Ring-H2 Domain	[U-13C; U-15N]	protein	1.05 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.1 mM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -2.36 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -2.36 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -2.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V87, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution Structure of the Ring-H2 Finger Domain of Mouse Deltex Protein 2
Miyamoto, K., Muto, Y., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Ring-H2 Finger Domain, : 1 : 2 : 43 entities Detail

Interaction partners 1. Ring-H2 Finger Domain, : 1 interactors Detail

Experiments performed 2 experiments Detail