BMRB: 11307

Solution Structure of the RING finger Domain of the human UbcM4-interacting Protein 4

Authors

Miyamoto, K., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

KIAA0161 protein

Entity

1. RING finger Domain (polymer, Thiol state: free and other bound), 94 monomers, 10254.79 Da Detail

GSSGSSGCKL CLGEYPVEQM TTIAQCQCIF CTLCLKQYVE LLIKEGLETA ISCPDAACPK QGHLQENEIE CMVAAEIMQR YKKLQFERSG PSSG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

10385.608 Da

Max. entity weight

10254.79 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS8:SG	2:ZN1:ZN
2	na	sing	1:CYS11:SG	2:ZN1:ZN
3	na	sing	1:CYS31:SG	2:ZN1:ZN
4	na	sing	1:CYS34:SG	2:ZN1:ZN
5	na	sing	1:CYS26:SG	2:ZN1:ZN
6	na	sing	1:CYS28:SG	2:ZN1:ZN
7	na	sing	1:CYS53:SG	2:ZN1:ZN
8	na	sing	1:CYS58:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 95.9 %, Completeness (bb): 93.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.9 % (1017 of 1061)	96.2 % (534 of 555)	96.1 % (391 of 407)	92.9 % (92 of 99)
Backbone	93.5 % (520 of 556)	93.2 % (179 of 192)	94.2 % (258 of 274)	92.2 % (83 of 90)
Sidechain	98.1 % (580 of 591)	97.8 % (355 of 363)	98.6 % (216 of 219)	100.0 % (9 of 9)
Aromatic	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (24 of 24)
Methyl	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (46 of 46)

1. RING finger Domain

GSSGSSGCKL CLGEYPVEQM TTIAQCQCIF CTLCLKQYVE LLIKEGLETA ISCPDAACPK QGHLQENEIE CMVAAEIMQR YKKLQFERSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.15mM RING finger Domain {U-13C,15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.1mM {ZnCl2;} 10%D2O

#	Name	Isotope labeling	Type	Concentration
1	RING finger Domain	[U-13C; U-15N]	protein	1.15 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.1 mM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.01 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WIM, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution Structure of the RING finger Domain of the human UbcM4-interacting Protein 4
Miyamoto, K., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. RING finger Domain, : 1 : 5 : 83 entities Detail

Interaction partners 1. RING finger Domain, : 6 interactors Detail

Experiments performed 2 experiments Detail