BMRB: 11308

Solution structure of the C2H2 zinc finger domain of the protein arginine N-methyltransferase 3 from Mus musculus

Authors

Miyamoto, K., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Protein arginine N-methyltransferase 3

Entity

1. C2H2 zinc finger domain (polymer, Thiol state: free and other bound), 121 monomers, 13772.23 Da Detail

GSSGSSGEPA HGRQHTPCLF CDRLFASAEE TFSHCKLEHQ FNIDSMVHKH GLEFYGYIKL INFIRLKNPT VEYMNSIYNP VPWEKDEYLK PVLEDDLLLQ FDVEDLYEPV STPFSSGPSS G

2. ZN (non-polymer), 65.409 Da

Total weight

13837.64 Da

Max. entity weight

13772.23 Da

Entity Connection

na 4 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:HIS34:NE2	2:ZN1:ZN
4	na	sing	1:HIS39:NE2	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 95.2 %, Completeness (bb): 93.1 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.2 % (1356 of 1424)	94.5 % (699 of 740)	96.8 % (545 of 563)	92.6 % (112 of 121)
Backbone	93.1 % (659 of 708)	90.9 % (219 of 241)	94.9 % (337 of 355)	92.0 % (103 of 112)
Sidechain	97.2 % (806 of 829)	96.2 % (480 of 499)	98.8 % (317 of 321)	100.0 % (9 of 9)
Aromatic	100.0 % (164 of 164)	100.0 % (82 of 82)	100.0 % (81 of 81)	100.0 % (1 of 1)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. C2H2 zinc finger domain

GSSGSSGEPA HGRQHTPCLF CDRLFASAEE TFSHCKLEHQ FNIDSMVHKH GLEFYGYIKL INFIRLKNPT VEYMNSIYNP VPWEKDEYLK PVLEDDLLLQ FDVEDLYEPV STPFSSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.14mM C2H2 zinc finger domain {U-13C,15N;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.1mM {ZnCl2;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	C2H2 zinc finger domain	[U-13C; U-15N]	protein	1.14 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.1 mM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.21 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WIR, Strand ID: A Detail

---

Release date

2011-08-18

Citation

Solution structure of the C2H2 zinc finger domain of the protein arginine N-methyltransferase 3 from Mus musculus
Miyamoto, K., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. C2H2 zinc finger domain, : 1 : 2 : 7 entities Detail

Interaction partners 1. C2H2 zinc finger domain, : 1 interactors Detail

Experiments performed 2 experiments Detail