BMRB: 11310

Solution structure of the C-terminal domain of mouse phosphoacetylglucosamine mutase (PAGM)

Authors

Yoneyama, M., Tochio, N., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

phosphoacetylglucosamine mutase

Entity

1. phosphoacetylglucosamine mutase (polymer, Thiol state: not present), 112 monomers, 11917.17 Da Detail

GSSGSSGAIY VDLPNRQLKV KVADRRVIST TDAERQAVTP PGLQEAINDL VKKYTLARAF VRPSGTEDIV RVYAEANSQE SADRLAYEVS LLVFQLAGGI GERPQPSGPS SG

Formula weight

11917.17 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.8 %, Completeness: 92.4 %, Completeness (bb): 91.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.4 % (1137 of 1231)	92.0 % (588 of 639)	93.5 % (447 of 478)	89.5 % (102 of 114)
Backbone	91.0 % (599 of 658)	90.7 % (206 of 227)	91.7 % (299 of 326)	89.5 % (94 of 105)
Sidechain	94.2 % (636 of 675)	92.7 % (382 of 412)	96.9 % (246 of 254)	88.9 % (8 of 9)
Aromatic	96.2 % (50 of 52)	96.2 % (25 of 26)	96.2 % (25 of 26)
Methyl	98.5 % (132 of 134)	98.5 % (66 of 67)	98.5 % (66 of 67)

1. C-terminal domain

GSSGSSGAIY VDLPNRQLKV KVADRRVIST TDAERQAVTP PGLQEAINDL VKKYTLARAF VRPSGTEDIV RVYAEANSQE SADRLAYEVS LLVFQLAGGI GERPQPSGPS SG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05), Details 1mM C-terminal domain of Phosphoacetylglucosamine mutase(PAGM) U-15N, {13C;} 20mM Phosphate Buffer {Na;} 300mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of Phosphoacetylglucosamine mutase(PAGM)	[U-13C; U-15N]	protein	1 mM
2	Phosphate Buffer Na	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	300 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.06 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.06 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.16 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WJW, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the C-terminal domain of mouse phosphoacetylglucosamine mutase (PAGM)
Yoneyama, M., Tochio, N., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. phosphoacetylglucosamine mutase, : 1 : 23 entities Detail

Experiments performed 2 experiments Detail