BMRB: 11311

Solution structure of the FAS1 domain of human transforming growth factor-beta induced protein IG-H3

Authors

Yoneyama, M., Tomizawa, T., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Transforming growth factor-beta induced protein IG-H3

Entity

1. Transforming growth factor-beta induced protein IG-H3 (polymer, Thiol state: not present), 146 monomers, 15329.33 Da Detail

GSSGSSGMGT VMDVLKGDNR FSMLVAAIQS AGLTETLNRE GVYTVFAPTN EAFRALPPRE RSRLLGDAKE LANILKYHIG DEILVSGGIG ALVRLKSLQG DKLEVSLKNN VVSVNKEPVA EPDIMATNGV VHVITNVLQP SGPSSG

Formula weight

15329.33 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 95.9 %, Completeness (bb): 94.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.9 % (1539 of 1604)	95.0 % (786 of 827)	97.6 % (611 of 626)	94.0 % (142 of 151)
Backbone	94.9 % (818 of 862)	93.0 % (280 of 301)	96.7 % (408 of 422)	93.5 % (130 of 139)
Sidechain	96.7 % (843 of 872)	95.6 % (503 of 526)	98.2 % (328 of 334)	100.0 % (12 of 12)
Aromatic	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (27 of 27)
Methyl	97.9 % (188 of 192)	97.9 % (94 of 96)	97.9 % (94 of 96)

1. FAS1 domain

GSSGSSGMGT VMDVLKGDNR FSMLVAAIQS AGLTETLNRE GVYTVFAPTN EAFRALPPRE RSRLLGDAKE LANILKYHIG DEILVSGGIG ALVRLKSLQG DKLEVSLKNN VVSVNKEPVA EPDIMATNGV VHVITNVLQP SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 0.39mM FAS1 domain U-15N, {13C;} 20mM d-Tris-HCl {(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	FAS1 domain	[U-13C; U-15N]	protein	0.39 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.15 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.15 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.35 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X3B, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the FAS1 domain of human transforming growth factor-beta induced protein IG-H3
Yoneyama, M., Tomizawa, T., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Transforming growth factor-beta induced protein IG-H3, : 1 : 7 : 113 entities Detail

Interaction partners 1. Transforming growth factor-beta induced protein IG-H3, : 3 interactors Detail

Experiments performed 2 experiments Detail