BMRB: 11312

Solution structure of the C2H2 type zinc-binding domain of human zinc finger protein 64, isoforms 1 and 2

Authors

Yoneyama, M., Koshiba, S., Tochio, N., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 64, isoforms 1

Entity

1. C2H2 type zinc-binding domain (polymer, Thiol state: free and other bound), 70 monomers, 7641.453 Da Detail

GSSGSSGHPE KCSECSYSCS SKAALRIHER IHCTDRPFKC NYCSFDTKQP SNLSKHMKKF HGDMSGPSSG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

7772.271 Da

Max. entity weight

7641.453 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS12:SG	2:ZN1:ZN
2	na	sing	1:CYS15:SG	2:ZN1:ZN
3	na	sing	1:HIS28:NE2	2:ZN1:ZN
4	na	sing	1:HIS32:NE2	2:ZN1:ZN
5	na	sing	1:CYS40:SG	2:ZN1:ZN
6	na	sing	1:CYS43:SG	2:ZN1:ZN
7	na	sing	1:HIS56:NE2	2:ZN1:ZN
8	na	sing	1:HIS61:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 82.9 %, Completeness: 84.1 %, Completeness (bb): 78.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.1 % (647 of 769)	85.0 % (345 of 406)	85.0 % (250 of 294)	75.4 % (52 of 69)
Backbone	78.9 % (325 of 412)	78.2 % (111 of 142)	79.9 % (163 of 204)	77.3 % (51 of 66)
Sidechain	89.8 % (378 of 421)	88.6 % (234 of 264)	92.9 % (143 of 154)	33.3 % (1 of 3)
Aromatic	97.0 % (64 of 66)	97.0 % (32 of 33)	97.0 % (32 of 33)
Methyl	100.0 % (24 of 24)	100.0 % (12 of 12)	100.0 % (12 of 12)

1. C2H2 type zinc-binding domain

GSSGSSGHPE KCSECSYSCS SKAALRIHER IHCTDRPFKC NYCSFDTKQP SNLSKHMKKF HGDMSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.25mM C2H2 type zinc-binding domain U-15N, {13C;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 50uM ZnCl2 {;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	C2H2 type zinc-binding domain	[U-13C; U-15N]	protein	1.25 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	50 uM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.53 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X5W, Strand ID: A Detail

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Release date

2011-08-18

Citation

Solution structure of the C2H2 type zinc-binding domain of human zinc finger protein 64, isoforms 1 and 2
Yoneyama, M., Koshiba, S., Tochio, N., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. C2H2 type zinc-binding domain, : 1 : 1 : 229 entities Detail

Interaction partners 1. C2H2 type zinc-binding domain, : 12 interactors Detail

Experiments performed 2 experiments Detail